Safety and immunogenicity of an oral inactivated whole-cell Pseudomonas aeruginosa vaccine administered to healthy human subjects.

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Title Safety and immunogenicity of an oral inactivated whole-cell Pseudomonas aeruginosa vaccine administered to healthy human subjects.
Author Cripps, Allan W; Peek, Keith; Dunkley, Margaret; Vento, Kevin; Marjason, Joanne K.; McIntyre, Madonna E; Sizer, Phil; Croft, Duncan; Sedlak-Weinstein, Lis
Journal Name Infection and Immunity
Year Published 2006
Place of publication Washington, DC
Publisher American Society for Microbiology
Abstract This study examines the safety and immunogenicity of an oral, whole-cell Pseudomonas aeruginosa vaccine administered to healthy volunteers. Thirty subjects received an oral dose of Pseudostat in two timed, measured doses with serological follow-up to 56 days postvaccination. Following vaccination, several individuals were identified as antibody responders for all three immunoglobulin (Ig) isotypes tested, specifically against whole-cell P. aeruginosa extract and outer membrane proteins F and I. The mean pooled lipopolysaccharide antigen-specific IgA showed the most significant and constant increases in titer postdose, with a similar increase in titer for whole-cell P. aeruginosa extract-specific IgA. The results demonstrated an increased phagocytic ability of the selected macrophage cell line in post vaccination sera. Furthermore a significant increase in intracellular macrophage killing of opsonized P. aeruginosa was also demonstrated (82% on day 14 postdose) in the presence of the postvaccination sera. The safety component of the study did not show any vaccine-attributable adverse effects in any of the subjects, as documented by clinical evidence, hematology, and biochemistry profiles. We conclude that Pseudostat is safe and immunogenic in humans at this dose and that further studies to determine the appropriate dosage and efficacy are needed. In our study, we have shown that the most significant and sustained responses to oral vaccination in human adult volunteers were serum IgA levels and that pooled sera collected postimmunization have an increased capacity to promote opsonophagocytotic killing of P. aeruginosa.
Peer Reviewed Yes
Published Yes
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Copyright Statement Copyright 2006 American Society for Microbiology. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal link for access to the definitive, published version.
Volume 74
Issue Number 2
Page from 968
Page to 974
ISSN 0019-9567
Date Accessioned 2006-06-19
Language en_AU
Research Centre Menzies Health Institute Qld; Molecular Basis of Disease
Faculty Griffith Health Faculty
Subject PRE2009-Medical and Health Sciences
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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