Rapid detection of airborne viruses by personal bioaerosol sampler combined with the PCR device.

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Title Rapid detection of airborne viruses by personal bioaerosol sampler combined with the PCR device.
Author Agranovski, Igor E; Safatov, A. S.; Sergeev, A. A.; Pyankov, Oleg; Petrishchenko, V. A.; Mikheev, M. V.; Sergeev, A.N.
Journal Name Atmospheric Environment
Editor Prof Brimblecombe
Year Published 2006
Place of publication Oxford, UK
Publisher Taylor & Francis Inc.
Abstract A new personal sampler had been previously developed and verified for monitoring of viable airborne viruses. The aims of this project were to investigate a possibility of the utilization of the polymerase chain reaction (PCR) method to speed up the time consuming analytical procedures and to evaluate a lower detection limit of the combined (sampler-PCR) device. Tenfold serial dilutions of the initial suspension of the Vaccinia virus were aerosolized in the chamber and airborne viruses were monitored by two simultaneously operating samplers. The results of monitoring were successfully obtained by a standard plaque assay (live microbes) and by the PCR method (total DNA). The corresponding calculations to identify the minimal detectable concentration in the ambient air were then performed. It was found that the minimal detectable concentration of airborne viruses in the ambient air depends on the sampling time. As demonstrated, such concentration should be at least 125×103 PFU m−3 for a sampling time of as short as 1 min. The detectable concentration decreases with the increase of the sampling time and reaches 25×103 and 10×103 PFU m−3 for 5 and 12.5 min of sampling respectively.
Peer Reviewed Yes
Published Yes
Publisher URI http://www.elsevier.com/wps/find/journaldescription.cws_home/246/description#description
Alternative URI http://dx.doi.org/10.1016/j.atmosenv.2006.02.023
Volume 40
Page from 3924
Page to 3929
ISSN 1352-2310
Date Accessioned 2007-03-16
Language en_AU
Faculty Faculty of Engineering and Information Technology
Subject PRE2009-Infectious Agents
URI http://hdl.handle.net/10072/11465
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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