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dc.contributor.convenorAustralia National Action Network (Bone and Joint Decade, Queensland)
dc.contributor.authorRenno, ACM
dc.contributor.authorMcDonnell, Ann
dc.contributor.authorLaakso, Liisa
dc.contributor.editorAustralia National Action Network
dc.date.accessioned2017-05-03T12:57:53Z
dc.date.available2017-05-03T12:57:53Z
dc.date.issued2006
dc.date.modified2007-09-11T06:46:11Z
dc.identifier.urihttp://hdl.handle.net/10072/14885
dc.description.abstractAbstract Background and Objective: Some studies have shown that low-level laser therapy (LLLT) is able to stimulate the osteogenesis of bone tissue, increasing osteoblast proliferation and accelerating fracture consolidation. It is also suggested that LLLT may have a biostimulatory effect in tumor cells. However, the mechanism by which LLLT acts on cells is not fully understood. The aim of this study was to investigate the effects of 670nm, 780 nm and 830 nm laser irradiations on cell proliferation of primary osteoblast (MC3T3) and malignant osteosarcoma (MG63) cell lines in vitro. Material and Methods: Neonatal, murine, calvarial, osteoblastic and human osteosarcoma cell lines were studied. A single laser irradiation was performed at three different wavelengths, at the energies of 0.5, 1, 5 and 10 J/cm2. Twenty-four hours after LLLT, cell proliferation and alkaline phosphatase assays were assessed. Results: Osteoblast proliferation increased significantly after 830nm laser irradiation (at 10 J/cm2) but decreased after 780nm laser irradiation (at 1, 5 and 10 J/cm2). Osteosarcoma cell proliferation increased significantly after 670nm (at 5 J/cm2) and 780nm laser irradiations (at 1, 5 and 10 J/cm2), but not after 830nm laser irradiation. ALP activity in the osteoblast line was increased after 830nm laser irradiation at 10J/cm2, whereas ALP activity in the osteosarcoma line was not altered, irrespective of laser wavelength or intensity. Conclusion: Based on the conditions of this study, we conclude that each cell line responds differently to specific wavelength and dose combinations. Further investigations are required to investigate the physiological mechanisms responsible for the contrasting outcomes obtained when using laser irradiation on cultured normal and malignant bone cells.
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAustralia National Action Network (Bone anf Joint Decade, Queensland)
dc.publisher.placeBrisbane, Queensland
dc.publisher.urihttp://www.bjd.org.au/docs/Programme.pdf
dc.publisher.urihttp://www.bjd.org.au/
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofconferencenameBone and Joint Decade (Multidisciplinary Research Conference), Queensland
dc.relation.ispartofconferencetitleThe Bone and Joint Decade, Queensland
dc.relation.ispartofdatefrom2005-10-21
dc.relation.ispartofdateto2005-10-21
dc.relation.ispartoflocationMater Hospital, Brisbane, Queensland
dc.rights.retentionY
dc.subject.fieldofresearchcode270102
dc.titleThe effects of 780nm laser irradiation on osteoblast and osteosarcoma cell proliferation
dc.typeConference output
dc.type.descriptionE3 - Conferences (Extract Paper)
dc.type.codeE - Conference Publications
gro.date.issued2006
gro.hasfulltextNo Full Text
gro.griffith.authorMcDonnell, Ann A.
gro.griffith.authorLaakso, Liisa


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    Contains papers delivered by Griffith authors at national and international conferences.

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