Expression of bone matrix protein mRNAs by primary and cloned cultures of the regenerative phenotype of human periodontal fibroblasts

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Title Expression of bone matrix protein mRNAs by primary and cloned cultures of the regenerative phenotype of human periodontal fibroblasts
Author Ivanovski, Saso; Haase, H.R.; Bartold, P.M.
Journal Name Journal of Dental Research
Year Published 2001
Place of publication US
Publisher International Association for Dental Research
Abstract The successful regeneration of periodontal tissues is dependent, in part, on the ability of cells to reconstitute the mineralized tissues of cementum and bone. The aim of the present study was to characterize regeneration-associated cells in terms of their ability to express mineralized tissue macromolecules. Following guided tissue regeneration, cell cultures were established from regenerating tissue, periodontal ligament, and gingiva. Additionally, these cells were transfected, and single-cell-derived clones were established. Following treatment with platelet-derived growth factor-BB and insulin-derived growth factor-1, the presence of mRNA for alkaline phosphatase, osteocalcin, bone sialoprotein, osteopontin, and bone morphogenetic proteins-2 and -4 was assessed. The three cell types expressed similar mRNA levels for alkaline phosphatase, bone morphogenetic protein-2, and bone morphogenetic protein-4, whereas the expression of osteopontin, osteocalcin, and bone sialoprotein was greater in the periodontal ligament and regenerating tissue fibroblasts compared with the gingival fibroblasts. The two growth factors did not affect the expression of any of the genes. This study has identified markers that correlate with the known ability of periodontal ligament and regenerating tissue-derived fibroblasts to facilitate regeneration of the mineralized tissues of the periodontium.
Peer Reviewed Yes
Published Yes
Publisher URI http://jdr.iadrjournals.org/
Copyright Statement Copyright 2001 International & American Associations for Dental Research (IADR/AADR). The attached file is reproduced here in accordance with the copyright policy of the publisher. Use hypertext link for access to journal's website.
Volume 80
Issue Number 7
Page from 1665
Page to 1671
ISSN 0022-0345
Date Accessioned 2007-07-04
Date Available 2008-12-24T03:17:50Z
Language en_AU
Research Centre Griffith Health Institute; Molecular Basis of Disease
Faculty Griffith Health Faculty
Subject Dentistry
URI http://hdl.handle.net/10072/15419
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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