Mitochondria transmit apoptosis signalling in cardiomyocyte-like cells and isolated hearts exposed to experimental ischemia-reperfusion injury

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Title Mitochondria transmit apoptosis signalling in cardiomyocyte-like cells and isolated hearts exposed to experimental ischemia-reperfusion injury
Author Neuzil, Jiri; Widen, Cecilia; Gellert, Nina; Cowie, Emma Angela; Zobalova, Renata; Dong, Lan-feng; Wang, Xiufang; Lidebjer, Caroline; Dalen, Helge; Headrick, John Patrick; Witting, Paul K.
Journal Name Redox Report
Year Published 2007
Place of publication London, United Kingdom
Publisher W. S. Maney & Son Ltd
Abstract Ischemia-reperfusion (I/R) is a condition leading to serious complications due to death of cardiac myocytes. We used the cardiomyocyte-like cell line H9c2 to study the mechanism underlying cell damage. Exposure of the cells to simulated I/R lead to their apoptosis. Over-expression of Bcl-2 and Bcl-xL protected the cells from apoptosis while over-expression of Bax sensitized them to programmed cell death induction. Mitochondria-targeted coenzyme Q (mitoQ) and superoxide dismutase both inhibited accumulation of reactive oxygen species (ROS) and apoptosis induction. Notably, mtDNA-deficient cells responded to I/R by decreased ROS generation and apoptosis. Using both in situ and in vivo approaches, it was found that apoptosis occurred during reperfusion following ischemia, and recovery was enhanced when hearts from mice were supplemented with mitoQ. In conclusion, I/R results in apoptosis in cultured cardiac myocytes and heart tissue largely via generation of mitochondria-derived superoxide, with ensuing apoptosis during the reperfusion phase.
Peer Reviewed Yes
Published Yes
Publisher URI http://www.maney.co.uk/search?fwaction=show&fwid=209
Alternative URI http://dx.doi.org/10.1179/135100007X200227
Copyright Statement Copyright 2007 Maney Publishing. The attached file is reproduced here in accordance with the copyright policy of the publisher.Please refer to the journal's website for access to the definitive, published version.
Volume 12
Issue Number 3
Page from 148
Page to 162
ISSN 1351-0002
Date Accessioned 2008-03-03
Date Available 2009-09-09T07:17:28Z
Language en_AU
Research Centre Griffith Health Institute; Heart Foundation Research Centre; Molecular Basis of Disease
Faculty Griffith Health Faculty
Subject PRE2009-Cell Physiology; PRE2009-Medical Biochemistry: Other
URI http://hdl.handle.net/10072/17784
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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