Additive and synergistic bactericidal activity of antibodies directed against minor outer membrane proteins of Neisseria meningitidis

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Title Additive and synergistic bactericidal activity of antibodies directed against minor outer membrane proteins of Neisseria meningitidis
Author Weynants, Vincent E.; Feron, Christiane M.; Goraj, Karine K.; Bos, Martine P.; Denoel, Philippe A; Verlant, Vincent G.; Tommassen, Jan; Peak, Ian; Judd, Ralph C; Jennings, Michael Paul; Poolman, Jan T
Journal Name Infection and Immunity
Editor Alison D. O'Brien
Year Published 2007
Place of publication Washington, DC
Publisher American Society for Microbiology
Abstract Neisseria meningitidis serogroup B is a major cause of bacterial meningitis in younger populations. The available vaccines are based on outer membrane vesicles obtained from wild-type strains. In children less than 2 years old they confer protection only against strains expressing homologous PorA, a major, variable outer membrane protein (OMP). We genetically modified a strain in order to eliminate PorA and to overproduce one or several minor and conserved OMPs. Using a mouse model mimicking children's PorA-specific bactericidal activity, it was demonstrated that overproduction of more than one minor OMP is required to elicit antibodies able to induce complement-mediated killing of strains expressing heterologous PorA. It is concluded that a critical density of bactericidal antibodies needs to be reached at the surface of meningococci to induce complement-mediated killing. With minor OMPs, this threshold is reached when more than one antigen is targeted, and this allows cross-protection.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1128/IAI.00411-07
Volume 75
Issue Number 11
Page from 5434
Page to 5442
ISSN 0019-9567
Date Accessioned 2007-10-16
Date Available 2014-02-28T02:57:46Z
Language en_US
Research Centre Institute for Glycomics
Faculty Griffith Health Faculty
Subject PRE2009-Medical Bacteriology; PRE2009-Preventive Medicine
URI http://hdl.handle.net/10072/18237
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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