Using a Bioaerosol Personal Sampler in combination with Real-time PCR Analysis for Rapid Detection of Airborne Viruses

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Title Using a Bioaerosol Personal Sampler in combination with Real-time PCR Analysis for Rapid Detection of Airborne Viruses
Author Pyankov, Oleg; Agranovski, Igor E; Pyankova, Olga; Mokhonova, Ekaterina; Mokhonov, Vlad; Safatov, Alexander S.; Khromykh, Alexander A.
Journal Name Environmental Microbiology
Year Published 2007
Place of publication Oxford
Publisher Blackwell Publishing
Abstract We have recently developed a new personal sampler and demonstrated its feasibility for detection of viable airborne microorganisms including bacteria, fungi and viruses. To accelerate the time-consuming analytical procedure involving 2–5 days of biological testing, we employed a real-time PCR protocol in conjunction with the personal sampler for collection of airborne viruses. The advantage of this approach is that if the presence of a particular pathogen in the air is detected by the PCR, the remaining collecting liquid can be further analysed by more time-consuming biological methods to estimate the number of airborne infectious/live microorganisms. As sampling of bioaerosols in natural environments is likely to be associated with substantial contamination by a range of microorganisms commonly existing in an ambient air, an investigation of the specificity of detection by targeted PCR analysis is required. Here we present the results of the study on the detection of Influenza virus in the ambient air contaminated with high concentrations of bacteria and fungi using real-time PCR protocol. The combined sampling PCR detection method was found to be fully feasible for the rapid (∼2.5 h) and highly specific (no cross-reactivity) identification of the labile airborne virus in the air containing elevated concentrations of other microorganisms.
Peer Reviewed Yes
Published Yes
Publisher URI
Alternative URI
Volume 9
Issue Number 4
Page from 992
Page to 1000
ISSN 1462-2912
Date Accessioned 2007-06-21
Date Available 2009-10-09T06:12:30Z
Language en_AU
Research Centre Atmospheric Environment Research Centre
Faculty Faculty of Science, Environment, Engineering and Technology
Subject PRE2009-Infectious Agents
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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