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dc.contributor.authorLintell, Szu-Min
dc.contributor.authorMaguire, David
dc.contributor.authorLintell, Nicholas
dc.contributor.authorMcCabe, Michael
dc.contributor.authorGriffiths, Lyn
dc.date.accessioned2017-05-03T11:46:34Z
dc.date.available2017-05-03T11:46:34Z
dc.date.issued2007
dc.identifier.issn00652598
dc.identifier.doi10.1007/978-0-387-71764-7_5
dc.identifier.urihttp://hdl.handle.net/10072/19102
dc.description.abstractCervical cancer is one of the world's major health issues. Despite many studies in this field, the carcinogenetic events of malignant conversion in cervical tumours have not been significantly characterised. The first aim of this project was to investigate the mutation status of the tumour suppressor gene- Phosphatase and Tension Homolog (PTEN)- in cervical cancer tissue. The second aim of this study was the analysis in the same cervical cancer tissue for aberrations in the mitochondrial electron transport chain subunit gene NDUFB8, which is localised to the same chromosomal contig as PTEN. The third aim was the evaluation of the potential therapeutic anti-cancer drug 2,4-Thiazolidinediones (TZDs) and its affect in regulating the PTEN protein in a cervical cancer cell line (HeLa). To approach the aims, paraffin-embedded cancerous cervical tissue and non-cancerous cervical tissue were obtained. DNA recovered from those tissues was then used to investigate the putative genomic changes regarding the NDUFB8 gene utilising SYBR Green I Real-Time PCR. The PTEN gene was studied via Dual-Labelled probe Real-Time PCR. To investigate the protein expression change of the PTEN protein, HeLa cells were firstly treated with different concentrations of 2,4-Thiazolidinediones and the level of PTEN protein expression was then observed utilising standard protein assays. Results indicated that there were putative copy-number changes between the cancerous cervical tissue and non-cancerous cervical tissue, with regard to the PTEN locus. This implies a potential gain of the PTEN gene in cancerous cervical tissue. With regards to normal cervical tissue versus cancerous cervical tissue no significant melting temperature differences were observed with the SYBR Green I Real-Time PCR in respect to the NDUFB8 gene. A putative up-regulation of PTEN protein was observed in TZD treated HeLa cells.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer
dc.publisher.placeNew York LLC
dc.publisher.urihttp://dx.doi.org/10.1007/978-0-387-71764-7
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom31
dc.relation.ispartofpageto36
dc.relation.ispartofjournalAdvances in Experimental Medicine and Biology
dc.relation.ispartofvolume599
dc.rights.retentionY
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode11
dc.titlePTEN and NDUFB8 aberrations in cervical cancer tissue
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2015-02-02T04:16:53Z
gro.hasfulltextNo Full Text
gro.griffith.authorMaguire, David J.
gro.griffith.authorMcCabe, Michael
gro.griffith.authorGriffiths, Lyn
gro.griffith.authorLintell, Nicholas
gro.griffith.authorLintell, Szu-Min K.


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