Crystallisation and preliminary X-ray diffraction analysis of the carbohydrate-recognizing domain (VP8*) of bovine rotavirus strain NCDV

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Title Crystallisation and preliminary X-ray diffraction analysis of the carbohydrate-recognizing domain (VP8*) of bovine rotavirus strain NCDV
Author Yu, Xing; Guillion, Annabel; Szyczew, Alexander Jeffery; Kiefel, Milton; Coulson, Barbara S.; von Itzstein, Mark; Blanchard, Helen
Journal Name Acta Crystallographica Section F - Structural Biology and Crystallization Communications
Year Published 2008
Place of publication Denmark
Publisher Wiley-Blackwell Munksgaard
Abstract Infectivity of rotavirus is dramatically enhanced by proteolytic cleavage of its outer layer VP4 spike protein into two function domains, VP8* and VP5*. The carbohydrate-recognising domain VP8* is proposed to bind sialic acid-containing host cell-surface glycans, followed by a series of subsequent virus-cell interactions. Live attenuated human and bovine rotavirus vaccine candidates to prevent gastroenteritis have been derived from bovine rotavirus strain NCDV. The NCDV VP8*64-224 was over-expressed, purified to homogeneity and crystallized in the presence of a N-acetylneuraminic acid derivative. X-ray diffraction data were collected to a resolution of 2.0 Å and the crystallographic structure of NCDV VP8*64-224 determined by molecular replacement.
Peer Reviewed Yes
Published Yes
Publisher URI http://journals.iucr.org/f/issues/2008/06/00/issconts.html
Volume F64
Page from 509
Page to 511
ISSN 1744-3091
Date Accessioned 2008-06-02
Date Available 2011-06-09T22:41:06Z
Language en_AU
Research Centre Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject PRE2009-Medical and Health Sciences
URI http://hdl.handle.net/10072/20267
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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