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dc.contributor.authorCowieson, NP
dc.contributor.authorListwan, P
dc.contributor.authorKurz, M
dc.contributor.authorAagaard, A
dc.contributor.authorRavasi, T
dc.contributor.authorWells, C
dc.contributor.authorHuber, T
dc.contributor.authorHume, DA
dc.contributor.authorKobe, B
dc.contributor.authorMartin, JL
dc.date.accessioned2017-09-26T22:00:19Z
dc.date.available2017-09-26T22:00:19Z
dc.date.issued2005
dc.date.modified2010-08-26T07:38:27Z
dc.identifier.issn1345-711X
dc.identifier.doi10.1007/s10969-005-0462-7
dc.identifier.urihttp://hdl.handle.net/10072/20923
dc.description.abstractWe investigated the parallel production in medium throughput of mouse proteins, using protocols that involved recombinatorial cloning, protein expression screening and batch purification. The methods were scaled up to allow the simultaneous processing of tens or hundreds of protein samples. Scale-up was achieved in two stages. In an initial study, 30 targets were processed manually but with common protocols for all targets. In the second study, these protocols were applied to 96 target proteins that were processed in an automated manner. The success rates at each stage of the study were similar for both the manual and automated approaches. Overall, 15 of the selected 126 target mouse genes (12%) yielded soluble protein products in a bacterial expression system. This success rate compares favourably with other protein screening projects, particularly for eukaryotic proteins, and could be further improved by modifications at the cloning step
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherKluwer Academic Publishers
dc.publisher.placeNetherlands
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom13
dc.relation.ispartofpageto20
dc.relation.ispartofissue1
dc.relation.ispartofjournalJournal of structural and functional genomics
dc.relation.ispartofvolume6
dc.rights.retentionN
dc.subject.fieldofresearchEnvironmental sciences
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchInformation and computing sciences
dc.subject.fieldofresearchcode41
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode46
dc.titlePilot studies on the parallel production of soluble mouse proteins in a bacterial expression system
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2005
gro.hasfulltextNo Full Text
gro.griffith.authorMartin, Jennifer


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