Pseudomonas aeruginosa-specific IgG1 and IgG2 subclasses in enhancement of pulmonary clearance following passive immunisation in the rat

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Title Pseudomonas aeruginosa-specific IgG1 and IgG2 subclasses in enhancement of pulmonary clearance following passive immunisation in the rat
Author Dunkley, Margaret L.; Rajyaguru, Sonal; McCue, Amanda; Cripps, Allan W.; Kyd, Jennelle M.
Journal Name FEMS Immunology and Medical Microbiology
Year Published 2003
Place of publication Netherlands
Publisher Elsevier BV
Abstract Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen, which causes serious debilitating infections in patients with compromised lung function. The mechanism by which P. aeruginosa is cleared from the lung is not fully defined, although our previous studies have established a role for cellular immunity in protection against P. aeruginosa infections. This study aimed to evaluate the role of P. aeruginosa-specific IgG in protection against P. aeruginosa in a rat model of acute pulmonary infection. Immunoaffinity chromatography was used to purify total rat IgG from rat immune serum (rats immunised with P. aeruginosa) and non-immune serum. Untreated recipient rats were injected intravenously with different concentrations of pure IgG prepared from serum of unimmunised rats (non-immune IgG) or from rats immunised intestinally with killed P. aeruginosa (immune IgG) and infected intratracheally with P. aeruginosa 18 h later. The protective capability of the purified IgG against P. aeruginosa was assessed by measurement of reduction in P. aeruginosa infection in the lung 4 h after instillation of bacteria. Enhanced bacterial clearance induced by IgG was determined to be dose-dependent with a 1 mg dose failing to enhance clearance, whereas 5 mg of immune IgG enhanced clearance from the airways and the lung tissue. Measurement of the IgG1, IgG2a and IgG2b isotypes in serum and the lung lavage following transfer of P. aeruginosa-specific IgG found that all three were present. These results demonstrate that anti-P. aeruginosa IgG can enhance bacterial clearance from the airways in an acute infection and identify an important role for IgG in acute respiratory infections caused by P. aeruginosa.
Peer Reviewed Yes
Published Yes
Publisher URI http://www.sciencedirect.com/science/journal/09288244
Alternative URI http://dx.doi.org/10.1016/S0928-8244(03)00176-7
Volume 39
Issue Number 1
Page from 37
Page to 44
ISSN 0928-8244
Date Accessioned 2006-06-19
Date Available 2009-03-02T06:51:10Z
Language en_AU
Research Centre Molecular Basis of Disease; Griffith Health Institute
Faculty Griffith Health Faculty
Subject PRE2009-Medical and Health Sciences
URI http://hdl.handle.net/10072/21565
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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