Validation and Quantitation of an in vitro M-cell model

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Title Validation and Quantitation of an in vitro M-cell model
Author Tyrer, Peter; Foxwell, A. Ruth; Kyd, Jennelle; Harvey, Matthew; Sizer, Phillip; Cripps, Allan W.
Journal Name Biochemical and Biophysical Research Communications
Year Published 2003
Place of publication United States
Publisher Academic Press
Abstract This study has evaluated an in vitro model of the follicle-associated epithelia that overlie Peyer's patches of the small intestine. The model shares many phenotypic characteristics of M cells in vivo. Co-cultures of the human adenocarcinoma cell line Caco-2 and freshly isolated Peyer's patch cells were established. Fluorescence microscopy and quantitative image analysis were used to validate the model against known markers of M-cell phenotype. Apical expression of alkaline phosphatase was down-regulated in co-cultures and villin was re-distributed from the apical membrane to the cytoplasm. α5β1 integrin was found on the apical surfaces of the monolayers and B and T lymphocytes integrated into the monolayers. Particle transport was temperature-dependent in co-cultures, indicating that a transcytotic route was responsible. This model provides opportunities to study factors that influence M-cell development, assess putative Peyer's patch targeting in oral vaccine technologies, and study intestinal uptake in vitro.
Peer Reviewed Yes
Published Yes
Publisher URI http://www.sciencedirect.com/science/journal/0006291X
Alternative URI http://dx.doi.org/10.1016/S0006-291X(02)02631-1
Volume 299
Page from 377
Page to 383
ISSN 0006-291X
Date Accessioned 2006-06-21
Date Available 2009-03-02T06:51:19Z
Language en_AU
Research Centre Molecular Basis of Disease; Griffith Health Institute
Faculty Griffith Health Faculty
Subject PRE2009-Medical and Health Sciences
URI http://hdl.handle.net/10072/21567
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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