Balancing shiftwork and life outside work: Do 12-h shifts make a difference?

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Title Balancing shiftwork and life outside work: Do 12-h shifts make a difference?
Author Loudoun, Rebecca Jane
Journal Name Applied Ergonomics
Editor Professor J Wilson, Professor j Parsons
Year Published 2008
Place of publication UK
Publisher Elsevier
Abstract Amounts of βA-activin, βC-activin, activin receptor subunits ActRIIA and ActRIIB mRNA, and βA- and βC-activin subunit protein immunoreactivity were investigated in male Lewis rats, either untreated or after 5 or 10 weeks of CCl4 treatment to induce cirrhosis. Apoptosis was assessed histologically and with an in situ cell death detection kit (TUNEL). Reverse transcription and polymerase chain reaction were used to evaluate mRNA levels. Activin βA- and βC-subunit immunoreactivity was studied by immunohistochemistry using specific monoclonal antibodies. Hepatocellular apoptosis (P<0.001), increased βA- and βC-activin mRNAs (three- to fourfold; P<0.01) and increased βA- and βC-activin tissue immunoreactivity were evident, whereas ActRIIA mRNA concentrations fell (30%; P<0.01) after 5 weeks of CCl4 treatment. The mRNA concentrations at 10 weeks were not significantly different from controls, despite extensive hepatic nodule formation. We conclude that the increased activin subunit expression is associated with apoptosis, rather than hepatic fibrosis and nodule formation.
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Copyright Statement Copyright 2008 Elsevier. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
Volume 39
Issue Number 5
Page from 572
Page to 579
ISSN 0003-6870
Date Accessioned 2008-06-25
Language en_AU
Faculty Griffith Business School
Subject PRE2009-Industrial Relations
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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