dc.contributor.author | Brown, J | |
dc.contributor.author | Walter, TS | |
dc.contributor.author | Carter, L | |
dc.contributor.author | Abrescia, NGA | |
dc.contributor.author | Aricescu, AR | |
dc.contributor.author | Batuwangala, TD | |
dc.contributor.author | Bird, LE | |
dc.contributor.author | Brown, N | |
dc.contributor.author | Chamberlain, PP | |
dc.contributor.author | Davis, SJ | |
dc.contributor.author | Dubinina, E | |
dc.contributor.author | Endicott, J | |
dc.contributor.author | Fennelly, JA | |
dc.contributor.author | Gilbert, RJC | |
dc.contributor.author | Harkiolaki, M | |
dc.contributor.author | Hon, WC | |
dc.contributor.author | Kimberley, F | |
dc.contributor.author | Love, CA | |
dc.contributor.author | Mancini, EJ | |
dc.contributor.author | Manso-Sancho, R | |
dc.contributor.author | Nichols, CE | |
dc.contributor.author | Robinson, RA | |
dc.contributor.author | Sutton, GC | |
dc.contributor.author | Schueller, N | |
dc.contributor.author | Sleeman, MC | |
dc.contributor.author | Stewart-Jones, GB | |
dc.contributor.author | Vuong, M | |
dc.contributor.author | Welburn, J | |
dc.contributor.author | Zhang, Z | |
dc.contributor.author | Stammers, DK | |
dc.contributor.author | Owens, RJ | |
dc.contributor.author | Jones, EY | |
dc.contributor.author | Harlos, K | |
dc.contributor.author | Stuart, DI | |
dc.date.accessioned | 2017-05-03T11:37:24Z | |
dc.date.available | 2017-05-03T11:37:24Z | |
dc.date.issued | 2003 | |
dc.date.modified | 2009-09-03T07:17:52Z | |
dc.identifier.issn | 0021-8898 | |
dc.identifier.doi | 10.1107/S0021889803002012 | |
dc.identifier.uri | http://hdl.handle.net/10072/22002 | |
dc.description.abstract | An initial tranche of results from day-to-day use of a robotic system for setting up 100 nl-scale vapour-diffusion sitting-drop protein crystallizations has been surveyed. The database of over 50 unrelated samples represents a snapshot of projects currently at the stage of crystallization trials in Oxford research groups and as such encompasses a broad range of proteins. The results indicate that the nanolitre-scale methodology consistently identifies more crystallization conditions than traditional hand-pipetting-style methods; however, in a number of cases successful scale-up is then problematic. Crystals grown in the initial 100 nl-scale drops have in the majority of cases allowed useful characterization of X-ray diffraction, either in-house or at synchrotron beamlines. For a significant number of projects, full X-ray diffraction data sets have been collected to 3 Šresolution or better (either in-house or at the synchrotron) from crystals grown at the 100 nl scale. To date, five structures have been determined by molecular replacement directly from such data and a further three from scale-up of conditions established at the nanolitre scale. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Wiley-Blackwell Munksgaard | |
dc.publisher.place | Denmark | |
dc.publisher.uri | http://www.wiley.com/bw/journal.asp?ref=0021-8898 | |
dc.relation.ispartofpagefrom | 315 | |
dc.relation.ispartofpageto | 318 | |
dc.relation.ispartofissue | 2 | |
dc.relation.ispartofjournal | Journal of Applied Crystallography | |
dc.relation.ispartofvolume | 36 | |
dc.subject.fieldofresearch | Mathematical sciences | |
dc.subject.fieldofresearch | Physical sciences | |
dc.subject.fieldofresearch | Engineering | |
dc.subject.fieldofresearchcode | 49 | |
dc.subject.fieldofresearchcode | 51 | |
dc.subject.fieldofresearchcode | 40 | |
dc.title | A procedure for setting up high-throughput nanolitre crystallization experiments. II. Crystallization results | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.rights.copyright | © 2003 Blackwell Munksgaard and the author[s]. The definitive version is available at www.interscience.wiley.com | |
gro.date.issued | 2003 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Love, Christopher A. | |