Bacillus okhensis sp. nov., a halotolerant and alkalitolerant bacterium from an Indian saltpan

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Title Bacillus okhensis sp. nov., a halotolerant and alkalitolerant bacterium from an Indian saltpan
Author Nowlan, Bianca; Dodia, Mital S.; Singh, Satya P.; Patel, Bharat Kumar
Journal Name International Journal of Systematic and Evolutionary Microbiology
Editor Dr. Peter Kaempfer (Editor-in-Chief)
Year Published 2006
Place of publication United Kingdom
Publisher Society for General Microbiology
Abstract A strictly aerobic, rod-shaped bacterium (0.6–0.8x2–3 µm), designated strain Kh10-101T, was isolated from a saltpan (22° 15' N, 69° 1' E) in the vicinity of Port Okha, India. The creamish pigmented colonies of strain Kh10-101T were round, flat and translucent with irregular margins and a smooth surface. The strain possessed up to three subpolar flagella, and was motile by a corkscrew motion. The strain grew optimally at 37 °C (temperature growth range 25–40 °C) in a complex glucose-containing medium with 5 % NaCl (NaCl growth range 0–10 %) at pH 9 (pH growth range pH 7–10), indicating that it was a mesophilic halotolerant alkaliphile. The strain was sensitive to lincomycin, meticillin, cefuroxime and cephalexin, but resistant to gentamicin, tetracycline and cotrimazine. Spores were not detected and cells were heat sensitive. The isolate metabolized a range of carbohydrates and hydrolysed casein, gelatin and starch. Growth was not observed on aromatic compounds, Tween 40 or Tween 80. Nitrate was not reduced and catalase was produced. Electron microscopic examination of thin sections revealed a single thick Gram-positive cell wall. The DNA G+C content was 41±1 mol%. Phylogenetic analyses of the 16S rRNA gene sequence revealed that strain Kh10-101T was a member of the sixth rRNA group of the genus Bacillus, which includes alkalitolerant, alkaliphilic and halotolerant species. The halotolerant obligate alkaliphile Bacillus krulwichiae is the closest relative of strain Kh10-101T (96 % similarity) but a number of phenotypic differences suggest that strain Kh10-101T (=JCM 13040T=ATCC BAA-1137T) should be designated the type strain of a new species, for which the name Bacillus okhensis sp. nov. is proposed.
Peer Reviewed Yes
Published Yes
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Volume 56
Issue Number 5
Page from 1073
Page to 1077
ISSN 1466-5026
Date Accessioned 2009-04-02
Language en_AU
Research Centre Queensland Micro and Nanotechnology Centre
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Medicinal and Biomolecular Chemistry
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1a

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