Role of thioredoxin-1 in apoptosis induction by α-tocopheryl succinate and TNF-related apoptosis-inducing ligand in mesothelioma cells
Author(s)
Freeman, Ruth E
Neuzil, Jiri
Griffith University Author(s)
Year published
2006
Metadata
Show full item recordAbstract
Malignant mesothelioma (MM) is a fatal type of cancer. We studied the role of the redox-active protein thioredoxin-1 (Trx-1) in apoptosis induced in MM cells and their non-malignant counterparts (Met-5A) by a-tocopheryl succinate (a-TOS) and TNF-related apoptosis-inducing ligand (TRAIL). MM cells were susceptible to a-TOS and less to TRAIL, while Met-5A cells were susceptible to TRAIL and resistant to a-TOS. MM cells expressed very low level of the Trx-1 protein, which was high in Met-5A cells, while the level of Trx-1 mRNA was similar in all cell lines. Downregulation of Trx-1 further sensitised Met-5A cells to TRAIL but ...
View more >Malignant mesothelioma (MM) is a fatal type of cancer. We studied the role of the redox-active protein thioredoxin-1 (Trx-1) in apoptosis induced in MM cells and their non-malignant counterparts (Met-5A) by a-tocopheryl succinate (a-TOS) and TNF-related apoptosis-inducing ligand (TRAIL). MM cells were susceptible to a-TOS and less to TRAIL, while Met-5A cells were susceptible to TRAIL and resistant to a-TOS. MM cells expressed very low level of the Trx-1 protein, which was high in Met-5A cells, while the level of Trx-1 mRNA was similar in all cell lines. Downregulation of Trx-1 further sensitised Met-5A cells to TRAIL but not to a-TOS. Our data suggest that the role of Trx-1 in apoptosis modulation is unrelated to its anti-oxidant properties.
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View more >Malignant mesothelioma (MM) is a fatal type of cancer. We studied the role of the redox-active protein thioredoxin-1 (Trx-1) in apoptosis induced in MM cells and their non-malignant counterparts (Met-5A) by a-tocopheryl succinate (a-TOS) and TNF-related apoptosis-inducing ligand (TRAIL). MM cells were susceptible to a-TOS and less to TRAIL, while Met-5A cells were susceptible to TRAIL and resistant to a-TOS. MM cells expressed very low level of the Trx-1 protein, which was high in Met-5A cells, while the level of Trx-1 mRNA was similar in all cell lines. Downregulation of Trx-1 further sensitised Met-5A cells to TRAIL but not to a-TOS. Our data suggest that the role of Trx-1 in apoptosis modulation is unrelated to its anti-oxidant properties.
View less >
Journal Title
FEBS Letters
Volume
580
Issue
11
Publisher URI
Copyright Statement
© 2006 Elsevier. Please refer to the journal's website for access to the definitive, published version.
Subject
Medicinal and biomolecular chemistry
Biochemistry and cell biology
Evolutionary biology
Cancer cell biology