dc.contributor.author | Gao, Jin | |
dc.contributor.author | L. Collard, Rachael | |
dc.contributor.author | Bui, Loan | |
dc.contributor.author | C. Herington, Adrian | |
dc.contributor.author | Nicol, David L. | |
dc.contributor.author | A. Clements, Judith | |
dc.date.accessioned | 2017-05-03T14:31:48Z | |
dc.date.available | 2017-05-03T14:31:48Z | |
dc.date.issued | 2007 | |
dc.date.modified | 2009-04-20T04:46:42Z | |
dc.identifier.issn | 0270-4137 | |
dc.identifier.doi | 10.1002/pros.20465 | |
dc.identifier.uri | http://hdl.handle.net/10072/22606 | |
dc.description.abstract | BACKGROUND. Prostate cancer (PCa) and bone cell interactions are critical in the metastatic phase. Kallikrein 4 (KLK4/hK4) is expressed in both PCa and mineralized tissues. We determined if KLK4/hK4 expression was associated with, and influenced by, the bone environment of metastatic PCa. METHODS. Immunohistochemistry, in vitro co-culture, cell migration, and attachment assays. RESULTS. hK4 was localized to tumor cells and osteoblasts in bone metastases. KLK4/hK4 increased in LNCaP and PC3 cells co-cultured with SaOs2 cells; SaOs2 KLK4/hK4 was unchanged. Co-culture did not affect cell proliferation but altered alkaline phosphatase activity/mRNA levels in SaOs2 cells. KLK4-transfected PC3 cells had increased migration towards SaOs2 conditioned medium and greater attachment to the bone-matrix proteins, collagens I and IV. CONCLUSIONS. hK4 expression and interaction with both tumor cells and osteoblasts suggests a role for hK4 in PCa bone metastasis. Whether this observation is unique to bone metastasis or reflects a role for hK4 in PCa metastasis generally is yet to be established. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Wiley-Liss,Inc. | |
dc.publisher.place | USA | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 348 | |
dc.relation.ispartofpageto | 360 | |
dc.relation.ispartofissue | 4 | |
dc.relation.ispartofjournal | The Prostate | |
dc.relation.ispartofvolume | 67 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Clinical Sciences | |
dc.subject.fieldofresearch | Oncology and Carcinogenesis | |
dc.subject.fieldofresearch | Paediatrics and Reproductive Medicine | |
dc.subject.fieldofresearchcode | 1103 | |
dc.subject.fieldofresearchcode | 1112 | |
dc.subject.fieldofresearchcode | 1114 | |
dc.title | Kallikrein 4 Is a Potential Mediator of Cellular Interactions Between Cancer Cells and Osteoblasts in Metastatic Prostate Cancer | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.date.issued | 2007 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Gao, Jin | |