Antioxidant Gene Expression in Preeclamptic Placentae: A Preliminary Investigation

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Title Antioxidant Gene Expression in Preeclamptic Placentae: A Preliminary Investigation
Author Vanderlelie, Jessica Joan; Gude, N.; Perkins, Anthony Vincent
Journal Name Placenta
Year Published 2008
Place of publication Holland
Publisher Elsevier
Abstract Oxidative stress has been implicated in the pathogenesis of preeclampsia. This study measured the relative mRNA expression of antioxidant proteins glutathione peroxidase 1 and 4, glutathione reductase, thioredoxin 1 and 2, thioredoxin reductase 1, thioredoxin peroxidase 3 and superoxide dismutase 1 and 2 in preeclamptic and non-preeclamptic placentae. Quantitative real-time PCR was conducted on placental mRNA isolated from preeclamptic and control patients. Cycle threshold numbers and fold differences were calculated as a measure of linear product amplification and used for comparison. The mRNA expression of glutathione reductase was significantly reduced (fold difference 0.41, p<0.05) in preeclamptic placenta when compared to controls while the expression of thioredoxin peroxidase 3 was significantly increased (fold difference 3.25, p<0.001) in the preeclamptic placentae. No significant difference in expression was observed for glutathione peroxidase 1 and 4, thioredoxin 1 and 2, thioredoxin reductase 1 and superoxide dismutase 1 and 2. These results suggest that it is the abnormal oxidative insult associated with preeclampsia not mRNA expression of antioxidant proteins that may be responsible for reduced antioxidant enzyme activity in preeclamptic placentae.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1016/j.placenta.2008.02.016
Volume 29
Issue Number 6
Page from 519
Page to 522
ISSN 0143-4004
Date Accessioned 2009-02-23
Language en_AU
Research Centre Griffith Health Institute
Faculty Griffith Health Faculty
Subject PRE2009-Biochemistry and Cell Biology
URI http://hdl.handle.net/10072/23374
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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