Olfactory Neuronal Cell Lines Generated by Retroviral Insertion of the N-myc Oncogene Display Different Developmental Phenotypes

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Title Olfactory Neuronal Cell Lines Generated by Retroviral Insertion of the N-myc Oncogene Display Different Developmental Phenotypes
Author MacDonald, Kelli Patricia; Mackay-Sim, Alan; Bushell, Gillian Robin; Bartlett, P.F.
Journal Name Journal of Neuroscience Research
Year Published 1996
Place of publication USA
Publisher John Wiley & Sons
Abstract Being genetically homogeneous, clonal cell lines are potentially important for investigating many aspects of cellular differentiation. We describe here the creation of clonal cell lines by immortalization of neuronal precursor cells from the adult mouse olfactory epithelium. Unlike neurons elsewhere in the vertebrate nervous system, the olfactory sensory neuron can be replaced throughout the lifespan of the animal. However, little is known about the molecular aspects of olfactory neurogenesis. Continuous cell lines were generated by retroviral transduction of the n-myc proto-oncogene into the mitotically active basal cells of the olfactory epithelium which give rise to the sensory neuron. Twenty-one clonal cell lines were produced which could be divided into three distinct morphological classes: one with flat, epithelial-like cells only; another with round, flat, and bipolar cells; and a third with large flat and large bipolar cells. These morphological classes had different patterns of intermediate filament expression, as shown by immunocytochemistry and immunoblot analysis. All cells in all cell lines expressed the intermediate filament protein vimentin. Most bipolar cells, but not other cell types, expressed neurofilament protein and in one morphological class the bipolar cells co-expressed neurofilament and glial fibrillary acidic protein. Several cell lines expressed mRNA for OMP, a marker of mature olfactory sensory neurons, and GOLF, a guanine nucleotide binding protein involved in olfactory sensory transduction. It is concluded that these cell lines were immortalized from sensory neuron precursors late in the lineage pathway. Other cell lines appear to have been immortalized at earlier stages in the lineage pathway. These cell lines therefore provide useful tools for the investigation of neuronal differentiation and sensory transduction in the olfactory epithelium.
Peer Reviewed Yes
Published Yes
Publisher URI http://www3.interscience.wiley.com/journal/34828/home
Alternative URI http://dx.doi.org/10.1002/(SICI)1097-4547(19960801)45:3<237::AID-JNR5>3.0.CO;2-E
Copyright Statement Copyright 1996 John Wiley & Sons, Ltd. Self-archiving of the author-manuscript version is not yet supported by this publisher. Please refer to the journal link for access to the definitive, published version or contact the author for more information.
Volume 45
Page from 237
Page to 247
ISSN 0360-4012
Date Accessioned 1997-01-01
Language en_AU
Research Centre Eskitis Institute for Drug Discovery
URI http://hdl.handle.net/10072/25734
Publication Type Article in Scholarly Refereed Journal
Publication Type Code c1

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