Nasal-associated lymphoid tissue and olfactory epithelium as portals of entry for Burkholderia pseudomallei in murine melioidosis

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Title Nasal-associated lymphoid tissue and olfactory epithelium as portals of entry for Burkholderia pseudomallei in murine melioidosis
Author Owen, Suzzanne; Batzloff, Michael; Chehrehasa, Fatemeh; Meedeniya, Adrian Cuda Banda; Casart Quintero, Yveth; Logue, Carie-Anne; Hirst, Robert G.; Peak, Ian; Mackay-Sim, Alan; Beacham, Ifor Rhys
Journal Name Journal of Infectious Diseases
Year Published 2009
Place of publication United States
Publisher University of Chicago Press
Abstract BACKGROUND: Burkholderia pseudomallei, the causative agent of melioidosis, is generally considered to be acquired via inhalation of dust or water droplets from the environment. In this study, we show that infection of the nasal mucosa is potentially an important portal of entry in melioidosis. METHODS: After intranasal inoculation of mice, infection was monitored by bioluminescence imaging and by immunohistological analysis of coronal sections. The bacterial loads in organ and tissue specimens were also monitored. RESULTS: Bioluminescence imaging showed colonization and replication in the nasal cavity, including the nasal-associated lymphoid tissue (NALT). Analysis of coronal sections and immunofluorescence microscopy further demonstrated the presence of infection in the respiratory epithelium and the olfactory epithelium (including associated nerve bundles), as well as in the NALT. Of significance, the olfactory epithelium and the brain were rapidly infected before bacteria were detected in blood, and a capsule-deficient mutant infected the brain without significantly infecting blood. CONCLUSIONS: These data suggest that the olfactory nerve is the route of entry into the brain and that this route of entry may be paralleled in cases of human neurologic melioidosis. This study focuses attention on the upper respiratory tract as a portal of entry, specifically focusing on NALT as a route for the development of systemic infection via the bloodstream and on the olfactory epithelium as a direct route to the brain.
Peer Reviewed Yes
Published Yes
Publisher URI http://www.journals.uchicago.edu/toc/jid/current
Alternative URI http://dx.doi.org/10.1086/599210
Copyright Statement Copyright 2009 by University of Chicago Press. The attached file is reproduced here in accordance with the copyright policy of the publisher. First published in The Journal of Geology. Please refer to the journal's website for access to the definitive, published version.
Volume 199
Issue Number 12
Page from 1761
Page to 1770
ISSN 0022-1899
Date Accessioned 2009-07-08
Language en_AU
Research Centre Eskitis Institute for Drug Discovery; Molecular Basis of Disease; Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Central Nervous System; Infectious Agents
URI http://hdl.handle.net/10072/25756
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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