Show simple item record

dc.contributor.authorVandez, Yanet
dc.contributor.authorDiehl, Gretchen E.
dc.contributor.authorVallance, Bruce A.
dc.contributor.authorGrassl, Guntram A.
dc.contributor.authorGuttman, Julian A.
dc.contributor.authorBrown, Nat F.
dc.contributor.authorRosenberger, Carrie M.
dc.contributor.authorLittman, Dan R.
dc.contributor.authorGros, Philippe
dc.contributor.authorBrett Finlay, B.
dc.date.accessioned2017-05-03T12:25:14Z
dc.date.available2017-05-03T12:25:14Z
dc.date.issued2008
dc.date.modified2009-11-26T09:45:34Z
dc.identifier.issn14625814
dc.identifier.doi10.1111/j.1462-5822.2008.01155.x
dc.identifier.urihttp://hdl.handle.net/10072/26918
dc.description.abstractHost resistance against Salmonella enterica serovar Typhimurium (S. Typhimurium) is mediated by natural resistance-associated macrophage protein 1 (Nramp1/Slc11a1). Nramp1 is critical to host defence, as mice lacking Nramp1 fail to control bacterial replication and succumb to low doses of S. Typhimurium. Despite this crucial role, the mechanisms underlying Nramp1's protective effects are unclear. Dendritic cells (DCs) that sample the intestinal lumen are among the first cells encountered by S. Typhimurium following oral infection and act as a conduit for S. Typhimurium to cross the intestinal epithelial barrier. We report that DCs, including intestinal, splenic and bone marrow-derived DCs (BMDCs), express Nramp1 protein. In the small intestine, Nramp1 expression is greater in a subset of DCs (CD11c+CD103-) characterized by the elevated expression of pro-inflammatory cytokines in response to bacterial products. While Nramp1 expression did not affect S. Typhimurium replication in BMDCs, infected Nramp1+/+ BMDCs and intestinal CD11c+CD103- DCs secreted more inflammatory cytokines (IL-6, IL-12 and TNF-a) than Nramp1-/-, suggesting that Nramp1 expression may promote a more rapid inflammatory response following infection. Collectively, these findings reveal a new role for DCs and Nramp1 in modulating the host inflammatory response to S. Typhimurium.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherBlackwell
dc.publisher.placeOxford
dc.publisher.urihttps://onlinelibrary.wiley.com/doi/10.1111/j.1462-5822.2008.01155.x
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1646
dc.relation.ispartofpageto1661
dc.relation.ispartofissue8
dc.relation.ispartofjournalCellular Microbiology
dc.relation.ispartofvolume10
dc.rights.retentionY
dc.subject.fieldofresearchMicrobiology not elsewhere classified
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchcode060599
dc.subject.fieldofresearchcode0605
dc.subject.fieldofresearchcode1108
dc.titleNramp1 expression by dendritic cells modulates inflammatory responses during Salmonella Typhimurium infection
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2008
gro.hasfulltextNo Full Text
gro.griffith.authorBrown, Nathaniel F.


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record