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dc.contributor.authorRahmat, Wibowo
dc.contributor.authorFischer, Joshua
dc.contributor.authorBrynn Hibbert, D.
dc.contributor.authorG. Gilbert, Robert
dc.contributor.authorJustin Gooding, J.
dc.contributor.editorJoseph Wang
dc.date.accessioned2017-05-03T16:58:23Z
dc.date.available2017-05-03T16:58:23Z
dc.date.issued2003
dc.date.modified2009-12-05T05:18:11Z
dc.identifier.issn10400397
dc.identifier.doi10.1002/elan.200302784
dc.identifier.urihttp://hdl.handle.net/10072/27109
dc.description.abstractAn enzyme electrode for glucose is described as a model system to demonstrate a fabrication method using latex aggregation and entrapment of enzyme. Electrosterically-stabilized latex particles synthesized by emulsion polymerization in batch from acrylic acid, methyl methacrylate and butyl acrylate, and glucose oxidase were coagulated together at pH 5.5 with ethanol. A platinum disk electrode dipped in the solution becomes coated with latex/enzyme. The relative thickness of the film and relative amount of enzyme may be controlled by the time the electrode is in contact with the solution. The enzyme was then immobilized by covalent attachment of amine groups to carboxylic moieties in the polymer using 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide hydrochloride and N-hydroxysuccinimide. Five minutes contact with the latex/enzyme solution and subsequent amide coupling, gave electrodes with a reproducibility of 5.7% RSD, a wide dynamic range (0-100 mM) and good storage properties.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley-VCH Verlag GmbH & Co. KGaA
dc.publisher.placeGermany
dc.relation.ispartofpagefrom1364
dc.relation.ispartofpageto1368
dc.relation.ispartofissue17
dc.relation.ispartofjournalElectroanalysis
dc.relation.ispartofvolume15
dc.subject.fieldofresearchAnalytical Chemistry
dc.subject.fieldofresearchPhysical Chemistry (incl. Structural)
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchcode0301
dc.subject.fieldofresearchcode0306
dc.subject.fieldofresearchcode0399
dc.titleUsing the Aggregation of Latex Polymers in the Fabrication of Reproducible Enzyme Electrodes
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2003
gro.hasfulltextNo Full Text
gro.griffith.authorFischer, Joshua


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