A Novel Corepressor, BCoR-L1, Represses Transcription Through an Interaction with CtBP

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Title A Novel Corepressor, BCoR-L1, Represses Transcription Through an Interaction with CtBP
Author Pagan, Julia K.; Arnold, Jeremy; Hanchard, Kim J.; Kumar, Raman; Bruno, Tiziana; Jones, Matthew J. K.; Richard, Derek J.; Forrest, Alistair; Spurdle, Amanda; Verdin, Eric; Crossley, Merlin; Fanciulli, Maurizio; Chenevix-Trench, Georgia; Young, David B.; Khanna, Kum Kum
Journal Name Journal of Biological Chemistry
Year Published 2007
Place of publication USA
Publisher American Society for Biochemistry and Molecular Biology
Abstract Corepressors play a crucial role in negative gene regulation and are defective in several diseases. BCoR is a corepressor for the BCL6 repressor protein. Here we describe and functionally characterize BCoR-L1, a homolog of BCoR. When tethered to a heterologous promoter, BCoR-L1 is capable of strong repression. Like other corepressors, BCoR-L1 associates with histone deacetylase (HDAC) activity. Specifically, BCoR-L1 coprecipitates with the Class II HDACs, HDAC4, HDAC5, and HDAC7, suggesting that they are involved in its role as a transcriptional repressor. BCoR-L1 also interacts with the CtBP corepressor through a CtBP-interacting motif in its amino terminus. Abrogation of the CtBP binding site within BCoR-L1 partially relieves BCoR-L1-mediated transcriptional repression. Furthermore, BCoR-L1 is located on the E-cadherin promoter, a known CtBP-regulated promoter, and represses the E-cadherin promoter activity in a reporter assay. The inhibition of BCoR-L1 expression by RNA-mediated interference results in derepression of E-cadherin in cells that do not normally express E-cadherin, indicating that BCoR-L1 contributes to the repression of an authentic endogenous CtBP target.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1074/jbc.M700246200
Volume 282
Issue Number 20
Page from 15248
Page to 15257
ISSN 0021-9258
Date Accessioned 2009-08-17
Language en_AU
Faculty Faculty of Science, Environment, Engineering and Technology
Subject PRE2009-Biochemistry and Cell Biology
URI http://hdl.handle.net/10072/27136
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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