AAA ATPases regulate membrane association of yeast oxysterol binding proteins and sterol metabolism

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Title AAA ATPases regulate membrane association of yeast oxysterol binding proteins and sterol metabolism
Author Wang, Penghua; Zhang, Yong; Li, Hongzhe; Chieu, Hai Kee; Munn, Alan L; Yang, Hongyuan
Journal Name EMBO journal
Editor Pernille Rorth
Year Published 2005
Place of publication United Kingdom
Publisher Nature Publishing Group
Abstract The yeast genome encodes seven oxysterol binding protein homologs, Osh1p–Osh7p, which have been implicated in regulating intracellular lipid and vesicular transport. Here, we show that both Osh6p and Osh7p interact with Vps4p, a member of the AAA (ATPases associated with a variety of cellular activities) family. The coiled-coil domain of Osh7p was found to interact with Vps4p in a yeast two-hybrid screen and the interaction between Osh7p and Vps4p appears to be regulated by ergosterol. Deletion of VPS4 induced a dramatic increase in the membrane-associated pools of Osh6p and Osh7p and also caused a decrease in sterol esterification, which was suppressed by overexpression of OSH7. Lastly, overexpression of the coiled-coil domain of Osh7p (Osh7pCC) resulted in a multivesicular body sorting defect, suggesting a dominant negative role of Osh7pCC possibly through inhibiting Vps4p function. Our data suggest that a common mechanism may exist for AAA proteins to regulate the membrane association of yeast OSBP proteins and that these two protein families may function together to control subcellular lipid transport.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1038/sj.emboj.7600764
Volume 24
Page from 2989
Page to 2999
ISSN 1460-2075
Date Accessioned 2009-09-02
Language en_AU
Research Centre Griffith Health Institute; Molecular Basis of Disease
Faculty Griffith Health Faculty
Subject PRE2009-Biochemistry and Cell Biology
URI http://hdl.handle.net/10072/27209
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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