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dc.contributor.authorKruger, MJ
dc.contributor.authorEngelbrecht, AM
dc.contributor.authorEsterhuyse, J
dc.contributor.authordu Toit, E
dc.contributor.authorvan Rooyen, J
dc.date.accessioned2017-05-03T15:31:39Z
dc.date.available2017-05-03T15:31:39Z
dc.date.issued2007
dc.identifier.issn0007-1145
dc.identifier.doi10.1017/S0007114507658991
dc.identifier.urihttp://hdl.handle.net/10072/27449
dc.description.abstractWe have previously shown that dietary red palm oil (RPO) supplementation improves functional recovery in hearts subjected to ischaemia-reperfusion. However, little knowledge exists concerning the effects of RPO supplementation of a high-cholesterol diet on ischaemia-reperfusion injury. The signalling mechanisms responsible for RPO's effects in the presence of cholesterol also remain to be elucidated. Therefore, the aim of the present study was to examine the effects of RPO, given with a high-cholesterol diet, on mitogen-activated protein kinase (MAPK) phosphorylation and apoptosis. Long-Evans rats were fed a control diet, a control diet containing 2 % cholesterol, or a control diet containing 2 % cholesterol and 7 g RPO per kg (CRPO) for 5 weeks. Hearts were excised and mounted on an isolated working heart perfusion apparatus. Cardiac function was measured after which hearts were freeze-clamped and used to assess MAPK phosphorylation and to evaluate apoptosis. Cholesterol supplementation caused a poor aortic output (AO) recovery compared with the control group (35絠(sem 6粩 v. 55素(sem 2絩 %), but when RPO was added, the percentage AO increased significantly. The cholesterol group's poor AO was associated with a significant increase in p38-MAPK phosphorylation, whereas the CRPO-supplemented group showed as significant reduction in p38-MAPK phosphorylation when compared with the cholesterol-supplemented group. This significant reduction in p38-MAPK was also associated with reduced apoptosis as indicated by significant reductions in caspase-3 and poly(ADP-ribose) polymerase cleavage.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent40796 bytes
dc.format.extent131587 bytes
dc.format.mimetypetext/plain
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherCambridge University Press
dc.publisher.placeCambridge, England
dc.publisher.urihttp://journals.cambridge.org/action/displayJournal?jid=BJN
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom653
dc.relation.ispartofpageto660
dc.relation.ispartofissue4
dc.relation.ispartofjournalBritish Journal of Nutrition
dc.relation.ispartofvolume97
dc.rights.retentionY
dc.subject.fieldofresearchAnimal production
dc.subject.fieldofresearchFood sciences
dc.subject.fieldofresearchCardiology (incl. cardiovascular diseases)
dc.subject.fieldofresearchNutrition and dietetics
dc.subject.fieldofresearchcode3003
dc.subject.fieldofresearchcode3006
dc.subject.fieldofresearchcode320101
dc.subject.fieldofresearchcode3210
dc.titleDietary red palm oil reduces ischaemia–reperfusion injury in rats fed a hypercholesterolaemic diet
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2007 The Authors. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.date.issued2015-05-31T22:17:12Z
gro.hasfulltextFull Text
gro.griffith.authorDu Toit, Eugene


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