Structural and functional diversity of novel coronin 1C (CRN2) isoforms in muscle

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Title Structural and functional diversity of novel coronin 1C (CRN2) isoforms in muscle
Author Xavier, Charles-Peter; Rastetter, Raphael H.; Stumpf, Maria; Rosentreter, André; Müller, Rolf; Reimann, Jens; Cornfine, Susanne; Linder, Stefan; Vliet, Vanessa van; Hofmann, Andreas; Morgan, Reginald O.; Fernandez, Maria-Pilar; Schröder, Rolf; Noegel, Angelika A.; Clemen, Christoph S.
Journal Name Journal of Molecular Biology
Year Published 2009
Place of publication Netherlands
Publisher Elsevier
Abstract Coronin 1C (synonyms: coronin-3, CRN2), a WD40 repeat-containing protein involved in cellular actin dynamics, is ubiquitously expressed in human tissues. Here, we report on the identification and functional characterization of two novel coronin 1C isoforms, referred to as CRN2i2 and CRN2i3, which also associate with F-actin. Analyses of the coronin 1C gene disclosed a single promoter containing binding sites for myogenic regulatory factors and an alternative first exon 1b present in intron 1, which give rise to the novel isoforms. Chromatin immunoprecipitation studies demonstrate MyoD binding to a region of the CRN2 gene, which contains a highly conserved E-box element in exon 1a. Gel-filtration assays suggest that the largest isoform 3 exists as a monomer, in contrast to isoform 1 and isoform 2 appearing as trimers. CRN2i3, which can be induced by MyoD, is exclusively expressed in well-differentiated myoblasts as well as in mature skeletal muscle tissue. In human skeletal muscle, CRN2i3 is a novel component of postsynaptic neuromuscular junctions and thin filaments of myofibrils. Together, our findings postulate a role for CRN2 isoforms in the structural and functional organization of F-actin in highly ordered protein complexes.
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Copyright Statement Copyright 2009 Elsevier. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
Volume 393
Issue Number 2
Page from 287
Page to 299
ISSN 0022-2836
Date Accessioned 2009-10-18
Language en_AU
Research Centre Eskitis Institute for Drug Discovery
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Analytical Biochemistry
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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