Exogenous activation of δ- and κ-opioid receptors affords cardioprotection in isolated murine heart.

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Title Exogenous activation of δ- and κ-opioid receptors affords cardioprotection in isolated murine heart.
Author Peart, Jason Nigel John; Gross, Garrett J.
Journal Name Basic Research in Cardiology
Year Published 2004
Place of publication Germany
Publisher Dr. Dietrich Steinkopff Verlag
Abstract Controversy exists regarding the relative roles of δ- and κ-opioid receptor activation as a potential cardioprotective mechanism. Furthermore, the function of opioid receptor activation in cardioprotection has not been examined in the murine heart. To this end, we employed various concentrations of selective δ- and κ-opioid receptor agonists in an isolated murine heart model undergoing 20 min global ischemia followed by 45 min reperfusion. Left-ventricular developed pressure (LVDP) returned to 50.7 ± 2.1% of baseline values in untreated hearts. Infusion of the non-selective opioid agonist, morphine (10 µM), lead to a marked improvement in post-ischemic contractile recovery where LVDP returned to 65.5 ± 2.4% of baseline function. The -opioid selective agonist BW373U86 hydrochloride elicited maximal protection at a concentration of 1 µM which afforded 63.9 ± 3.4% recovery of LVDP. This effect was blocked by the δ-opioid selective antagonist, BNTX. Furthermore, administration of the κ-opioid selective agonist U50,488 (1 µM) produced a marked improvement in contractile recovery leading to a 72.5 ± 5.3% recovery of LVDP. This degree of protection was also abolished by the δ-opioid receptor antagonist, nor-BNI. No differences were noted in LDH efflux from post-ischemic hearts. These data suggest that exogenous activation of δ- and κ-opioid receptors afford protection against myocardial stunning in the isolated murine heart, an effect attenuated by selective receptor antagonists.
Peer Reviewed Yes
Published Yes
Publisher URI http://www.springer.com/steinkopff/kardiologie/journal/395
Alternative URI http://dx.doi.org/10.1007/s00395-003-0430-y
Volume 99
Page from 29
Page to 37
ISSN 0300-8428
Date Accessioned 2006-07-26
Date Available 2009-12-21T06:45:05Z
Language en_AU
Research Centre Griffith Health Institute; Heart Foundation Research Centre
Faculty Griffith Health Faculty
Subject PRE2009-Clinical Pharmacology and Therapeutics
URI http://hdl.handle.net/10072/27864
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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