Methods for the identification of differentially expressed genes in human post-mortem brain
Author(s)
Mayfield, RD
Liu, JW
Randall, PK
Lewohl, JM
Dodd, PR
Harris, RA
Griffith University Author(s)
Year published
2003
Metadata
Show full item recordAbstract
Microarrays can be used to monitor the expression of thousands of genes simultaneously. This technique requires high-quality RNA which can be extracted from a variety of tissues and cells including post-mortem human brain. Given the vast amount of information obtained from microarray studies, it is critical to establish valid analysis techniques to identify differentially expressed genes. This technical report describes the basic methodology and analyses used to identify such genes in human post-mortem brain tissue.Microarrays can be used to monitor the expression of thousands of genes simultaneously. This technique requires high-quality RNA which can be extracted from a variety of tissues and cells including post-mortem human brain. Given the vast amount of information obtained from microarray studies, it is critical to establish valid analysis techniques to identify differentially expressed genes. This technical report describes the basic methodology and analyses used to identify such genes in human post-mortem brain tissue.
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Journal Title
Methods
Volume
31
Issue
4
Subject
Clinical sciences