Characterisation of oxidized recombinant human galectin-1

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Title Characterisation of oxidized recombinant human galectin-1
Author Scott, Stacy; Bugarcic, Andrea; Blanchard, Helen
Journal Name Protein and Peptide Letters
Year Published 2009
Place of publication USA
Publisher Bentham Science Publishers
Abstract Oxidized human galectin-1 plays a role in the immune response to injured axons. Over-expression of galectin-1 by cancer, in combination with cancer associated oxidative stress suggests oxidized human galectin-1 may also play a role(s) in tumourigenesis. Here we generate milligram quantities of oxidized human galectin-1 and undertake biophysical characterization. The protein adopts a number of different states. Two separable oxidized forms are identified that exist as largely mono-disperse solutions at higher milligram/ml concentrations. The presence of disulphide bonds is confirmed for these two protein forms, as is their change in overall shape and loss of lectin activity. Our studies lead to production of a particular mono-disperse oxidized human galectin-1 species that is anticipated most optimal for investigations requiring milligram/ml concentrations such as X-ray crystallography.
Peer Reviewed Yes
Published Yes
Publisher URI
Alternative URI
Volume 16
Issue Number 10
Page from 1249
Page to 1255
ISSN 0929-8665
Date Accessioned 2009-10-01
Language en_AU
Research Centre Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Biochemistry and Cell Biology
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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