Facile construction of unmarked deletion mutants in Burkholderia pseudomallei using sacB counter-selection in sucrose-resistant and sucrose-sensitive isolates

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Title Facile construction of unmarked deletion mutants in Burkholderia pseudomallei using sacB counter-selection in sucrose-resistant and sucrose-sensitive isolates
Author Logue, Carie-Anne; Peak, Ian; Beacham, Ifor Rhys
Journal Name Journal of Microbiological Methods
Year Published 2009
Place of publication Netherlands
Publisher Elsevier
Abstract Burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic or emerging world-wide. Here we report unmarked allele-replacement mutagenesis using efficient sacB counter-selection. Despite being genotypically sacB(+), most commonly used B. pseudomallei strains are sucrose-resistant and efficient sacB counter-selection is demonstrated in both resistant and sensitive strains.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1016/j.mimet.2008.12.007
Copyright Statement Copyright 2009 Elsevier. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
Volume 76
Issue Number 3
Page from 320
Page to 323
ISSN 0167-7012
Date Accessioned 2009-07-07
Date Available 2010-01-29T06:18:32Z
Language en_AU
Research Centre Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Immunology; Infectious Agents; Medical Bacteriology
URI http://hdl.handle.net/10072/28519
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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