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dc.contributor.authorLaohavisit, Anuphon
dc.contributor.authorMortimer, Jennifer C
dc.contributor.authorDemidchik, Vadim
dc.contributor.authorCoxon, Katy M
dc.contributor.authorStancombe, Matthew A
dc.contributor.authorMacpherson, Neil
dc.contributor.authorBrownlee, Colin
dc.contributor.authorHofmann, Andreas
dc.contributor.authorWebb, Alex AR
dc.contributor.authorMiedema, Henk
dc.contributor.authorBattey, Nicholas H
dc.contributor.authorDavies, Julia M
dc.date.accessioned2017-05-03T15:19:39Z
dc.date.available2017-05-03T15:19:39Z
dc.date.issued2009
dc.date.modified2010-06-30T06:44:39Z
dc.identifier.issn1040-4651
dc.identifier.doi10.1105/tpc.108.059550
dc.identifier.urihttp://hdl.handle.net/10072/28525
dc.description.abstractRegulation of reactive oxygen species and cytosolic free calcium ([Ca2+]cyt) is central to plant function. Annexins are small proteins capable of Ca2+-dependent membrane binding or membrane insertion. They possess structural motifs that could support both peroxidase activity and calcium transport. Here, a Zea mays annexin preparation caused increases in [Ca2+]cyt when added to protoplasts of Arabidopsis thaliana roots expressing aequorin. The pharmacological profile was consistent with annexin activation (at the extracellular plasma membrane face) of Arabidopsis Ca2+-permeable nonselective cation channels. Secreted annexins could therefore modulate Ca2+ influx. As maize annexins occur in the cytosol and plasma membrane, they were incorporated at the intracellular face of lipid bilayers designed to mimic the plasma membrane. Here, they generated an instantaneously activating Ca2+-permeable conductance at mildly acidic pH that was sensitive to verapamil and Gd3+ and had a Ca2+-to-K+ permeability ratio of 0.36. These results suggest that cytosolic annexins create a Ca2+ influx pathway directly, particularly during stress responses involving acidosis. A maize annexin preparation also demonstrated in vitro peroxidase activity that appeared independent of heme association. In conclusion, this study has demonstrated that plant annexins create Ca2+-permeable transport pathways, regulate [Ca2+]cyt, and may function as peroxidases in vitro.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Society of Plant Biologists
dc.publisher.placeUnited States
dc.publisher.urihttp://www.plantcell.org/
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom479
dc.relation.ispartofpageto493
dc.relation.ispartofjournalPlant Cell
dc.relation.ispartofvolume21
dc.rights.retentionY
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchAnalytical biochemistry
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchPlant biology
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode310101
dc.subject.fieldofresearchcode3105
dc.subject.fieldofresearchcode3108
dc.titleZea mays annexins modulate cytosolic free Ca2+ and generate a Ca2+-permeable conductance
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2009 American Society of Plant Biologists. Self-archiving of the author-manuscript version is not yet supported by American Scientific Publishers. Please refer to the journal link for access to the definitive, published version or contact the authors for more information.
gro.date.issued2009
gro.hasfulltextNo Full Text
gro.griffith.authorHofmann, Andreas


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