Effect of gonadotropin-releasing hormone agonist and antagonist on proliferation and apoptosis of human luteinized granulosa cells
Author(s)
M. Vitale, Alejandra
Abramovich, Dalhia
C. Peluffo, Marina
Meresman, Gabriela
Tesone, Marta
Griffith University Author(s)
Year published
2006
Metadata
Show full item recordAbstract
The GnRH agonist leuprolide acetate (LA) inhibited DNA synthesis in epidermal growth factor-stimulated human granulosa luteinized cell cultures. This effect was blocked by the prior addition of a GnRH antagonist antide (ANT), and this compound per se was able to produce a stimulatory effect of DNA synthesis on basal conditions. Leuprolide acetate produced an increase in the percentage of apoptotic cells, and when these two factors were co-incubated, ANT blocked the apoptotic effect produced by LA.The GnRH agonist leuprolide acetate (LA) inhibited DNA synthesis in epidermal growth factor-stimulated human granulosa luteinized cell cultures. This effect was blocked by the prior addition of a GnRH antagonist antide (ANT), and this compound per se was able to produce a stimulatory effect of DNA synthesis on basal conditions. Leuprolide acetate produced an increase in the percentage of apoptotic cells, and when these two factors were co-incubated, ANT blocked the apoptotic effect produced by LA.
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Journal Title
Fertility and Sterility
Volume
85
Issue
4
Subject
Cell Development, Proliferation and Death
Clinical Sciences
Paediatrics and Reproductive Medicine
Public Health and Health Services