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The expression of chicken interleukin 2 in Pichia yeast

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Title The expression of chicken interleukin 2 in Pichia yeast
Author Jian, Xu; Fan, Yongjun; Long, Li; Wangjun, Wan; Jianrong, Li; Lian, Yu
Journal Name Journal of Xi'an Jiaotong University (Medical Sciences)
Year Published 2004
Place of publication China
Publisher Xi'an Jiaotong Daxue
Abstract Objective 　To express recombinant chicken interleukin 2 ( IL22) in Pichia yeast eukaryotic cell expression system for a massive preparation of chicken IL22. Methods 　Spleen lymphocytes taken from Xiaoshan chicken were activated by ConA in vitro. Chicken IL22 gene was amplified and cloned into pPIC9. The pPIC92IL22 was transformed into Pichia yeast by electroporation. The recombinants were induced by methanol for the expression of recombinant protein. Result s 　The protein was examined with SDS2PAGE and Western bloting. A specific molecular weight band at about 30 ku appeared. Conclusion 　 The recombinant protein of chicken IL22 has been expressed in Pichia yeast .
Peer Reviewed No
Published Yes
Publisher URI http://en.cnki.com.cn/Article_en/CJFDTOTAL-XAYX200404008.htm
Volume 25
Issue Number 4
Page from 339
Page to 342
ISSN 1671-8259
Date Accessioned 2010-02-19
Date Available 2010-02-26T06:37:21Z
Language en_AU
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Biomedical Engineering
URI http://hdl.handle.net/10072/28971
Publication Type Non Refereed Journal Articles
Publication Type Code c2

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