Analytical Approaches Towards the Structural Characterization of Microbial Wall Glycopolymers

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Title Analytical Approaches Towards the Structural Characterization of Microbial Wall Glycopolymers
Author Grice, Darren; Wilson, Jenny
Book Title Microbial Glycobiology: Structures, Relevance and Applications
Editor Anthony P. Moran, Otto Holst, Patrick J. Brennan, Mark von Itzstein
Year Published 2009
Place of publication Oxford, UK
Publisher Academic Press
Abstract Outlined in this chapter are strategies for the use of the analytical techniques of nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) towards the elucidation of the structures of microbial wall glycans. Specifically, the determination of the structure of capsular polysaccharide, core oligosaccharide or O-antigen utilizing 1D, 1D selective and 2D homonuclear and heteronuclear NMR experiments and database resources is presented. Described is the application of high resolution magic angle spinning techniques for studying glycopolymer structures. MS sample derivatization strategies along with associated gas chromatography-mass spectrometry, sugar, linkage, and absolute configuration analyses are outlined. The use of fast atom bombardment, matrix-assisted laser desorption ionization and electrospray-mass spectrometry ionization techniques along with their associated mass analysers are presented for characterizing oligosaccharide, lipid A, lipooligosaccahride, ipopolysaccharide and capsular polysaccharide glycan structures.
Peer Reviewed Yes
Published Yes
Publisher URI
Copyright Statement Self-archiving is not yet supported by this publisher. Please refer to the publisher's website or contact the author(s) for more information.
Edition First
Chapter Number 13
Page from 233
Page to 252
ISBN 978-0-12-374546-0
Date Accessioned 2010-02-02
Language en_US
Research Centre Institute for Glycomics
Faculty Griffith Health Faculty
Subject Characterisation of Biological Macromolecules
Publication Type Book Chapters
Publication Type Code b1

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