Design and evaluation of a new device for fungal spore aerosolization for laboratory applications

There are no files associated with this record.

Title Design and evaluation of a new device for fungal spore aerosolization for laboratory applications
Author Scheermeyer, Elly; Agranovski, Igor E
Journal Name Journal of Aerosol Science
Year Published 2009
Place of publication United Kingdom
Publisher Elsevier
Abstract Fungi havesignificanthealtheffectsvaryingfromseriousallergicreactionsfrominhalationto toxic effectsandevenfatalinfections.Indoorandoutdoorsamplingmayidentifypotentially contaminated environments,butsamplingtechniquesneedrefiningtosetrisklevelsregarding occupational exposureortoassessandanalysesuddenoutbreaksoffungi.Aslittleisknown of thesporulationanddispersaldynamicsoffungi,wehaveexperimentedwithaerosolizing fungal sporesfromculturesgrowninstandard9cmPetridishesfor3–10husinganewdevice that incorporatesvibration.Weevaluatedtheeffectoftheairflowandfrequencyonaquan- tity andpatternofsporesaerosolized.Frequencyhadpositive,negativeornoeffect.Positive quantitative effectswerenotedin Aspergillus versicolor at 50Hz,in Aspergillus niger at 400Hz and at100–400Hzin Rhizopus. Aerosolizationof Aspergillus species droppedsignificantlyin the first20minandthenstabilisedfor2.5–3h.Afterthistimeperiod,thegenerationprocess became highlyunstablewithlargequantitiesofsmallparticlesproduced.Itcouldbeexplained by appearanceofcracksonthenutrientsurfaceduetodesiccationwithcorrespondingrelease of particlesofnutrienttotheaircarrier.For Rhizopus, theinitialsteepdecreaseinparticle generation forthefirst20minoftheprocesswasfollowedbyquitestablesporeproductionfor the following10hofprocessoperation.Therewerenocracksobservedonthenutrientsurface for theentire10hoperationofthedevice.Theairflowalsoaffectstheefficiencyofthespore release. Itwasfoundthatincreaseintheairflowwasassociatedwithmoreefficientsporulation from thenutrientdish.Ontheotherhand,thesizedistributioncurveoftheairbornespores produced athigherairflowswascorrespondinglyslightlyshiftedtowardslargersizes,which is relatedtolessefficientlooseningofsporesandcorrespondingincreaseinconcentrationof clumps consistingofmorethanonespore.Also,itwasfoundthatincubationoffungalcultures for extendedperiods(2–6weeks)ledtowardsproductionoflargernumbersofairbornefungal spores.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1016/j.jaerosci.2009.06.003
Volume 40
Issue Number 10
Page from 879
Page to 889
ISSN 0021-8502
Date Accessioned 2010-04-21
Date Available 2010-06-24T05:22:20Z
Language en_AU
Research Centre Atmospheric Environment Research Centre
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Chemical Engineering
URI http://hdl.handle.net/10072/30657
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

Brief Record

Griffith University copyright notice