Rates of Platination of AG and GA containing double-stranded oliqonucleotides:effect of chloride concentration.

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Title Rates of Platination of AG and GA containing double-stranded oliqonucleotides:effect of chloride concentration.
Author Davies, Murray; Berners-Price, Sue; Hambley, Trevor W.
Journal Name Journal of Inorganic Biochemistry
Year Published 2000
Place of publication USA
Publisher Elsevier Science
Abstract The kinetics of the reactions between 15N-labelled cisplatin and 14-base pair duplex oligonucleotides with either 52-AG-32 or 52-GA-32 groupings as the principal platination site are examined in the presence of 6080 mM chloride by [1H,15N] HSQC 2D NMR spectroscopy. The presence of chloride at these concentrations results in a five-fold decrease in the rate of hydrolysis of cisplatin to cis-[PtCl(NH3)2(OH2)]+ and a two- to twenty-fold decrease in the rate of monofunctional adduct formation. The effects on the rate of closure from monofunctional to bifunctional adducts are less well established but some of these rates appear not to be significantly reduced by the presence of added chloride. The results provide a caution that the use of chloride to quench platination reactions may not be fully effective.
Peer Reviewed Yes
Published Yes
Publisher URI http://www.elsevier.com/wps/find/journaldescription.cws_home/505772/description#description
Copyright Statement Copyright 2000 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online - use hypertext links.
Volume 79
Page from 167
Page to 172
ISSN 0162-0134
Date Accessioned 2001-01-01
Language en_AU
Research Centre Institute for Glycomics
Subject Science & Technology
URI http://hdl.handle.net/10072/3213
Publication Type Article in Scholarly Refereed Journal
Publication Type Code c1

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