Potential of Sodium Monofluoroacetate (1080) and Fluorocitrate to Bind to the Estrogen Receptor
Author(s)
Tremblay, Louis
Fisher, Penny
Leusch, Fred
Griffith University Author(s)
Year published
2004
Metadata
Show full item recordAbstract
Sodium monofluoroacetate (1080), a vertebrate pesticide used in New Zealand, and its metabolite fluorocitrate, were tested to determine whether the pesticide could promote an estrogenic response by binding to a mammalian estrogen receptor (ER). ER preparations were isolated from sheep uterine tissue and used in a competitive binding assay. The assay was validated by generating displacement curves for the natural estrogen (E2) and known environmental estrogens like o,p'-DDT and 4-nonylphenol. The known estrogenic pesticide o,p'-DDT showed significant displacement of E2 while neither 1080 nor its active metabolite fluorocitrate ...
View more >Sodium monofluoroacetate (1080), a vertebrate pesticide used in New Zealand, and its metabolite fluorocitrate, were tested to determine whether the pesticide could promote an estrogenic response by binding to a mammalian estrogen receptor (ER). ER preparations were isolated from sheep uterine tissue and used in a competitive binding assay. The assay was validated by generating displacement curves for the natural estrogen (E2) and known environmental estrogens like o,p'-DDT and 4-nonylphenol. The known estrogenic pesticide o,p'-DDT showed significant displacement of E2 while neither 1080 nor its active metabolite fluorocitrate had any effect on ER binding. Sodium monofluoroacetate and fluorocitrate are unlikely to promote estrogenic or anti-estrogenic effects by binding to the ER.
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View more >Sodium monofluoroacetate (1080), a vertebrate pesticide used in New Zealand, and its metabolite fluorocitrate, were tested to determine whether the pesticide could promote an estrogenic response by binding to a mammalian estrogen receptor (ER). ER preparations were isolated from sheep uterine tissue and used in a competitive binding assay. The assay was validated by generating displacement curves for the natural estrogen (E2) and known environmental estrogens like o,p'-DDT and 4-nonylphenol. The known estrogenic pesticide o,p'-DDT showed significant displacement of E2 while neither 1080 nor its active metabolite fluorocitrate had any effect on ER binding. Sodium monofluoroacetate and fluorocitrate are unlikely to promote estrogenic or anti-estrogenic effects by binding to the ER.
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Journal Title
Australasian Journal of Ecotoxicology
Volume
10
Issue
2
Publisher URI
Subject
Environmental Impact Assessment
Chemical Sciences
Environmental Sciences
Biological Sciences