Expression analysis of a human hepatic cell line in response to palmitate

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Title Expression analysis of a human hepatic cell line in response to palmitate
Author Swagell, Christopher D.; Henly, Debra; Morris, C. Phillip
Journal Name Biochemical and Biophysical Research Communications
Year Published 2005
Place of publication United States
Publisher Academic Press
Abstract Saturated fat plays a role in common debilitating diseases such as obesity, type 2 diabetes, and coronary heart disease. It is also clear that certain fatty acids act as regulators of metabolism via both direct and indirect signalling of target tissues. As the molecular mechanisms of saturated fatty acid signalling in the liver are poorly defined, hepatic gene expression analysis was undertaken in a human hepatocyte cell line after incubation with palmitate. Profiling of mRNA expression using cDNA microarray analysis revealed that 162 of approximately 18,000 genes tested were differentially expressed after incubation with palmitate for 48 h. Altered transcription profiles were observed in a wide variety of genes, including genes involved in lipid and cholesterol transport, cholesterol catabolism, cell growth and proliferation, cell signalling, β-oxidation, and oxidative stress response. While palmitate signalling has been examined in pancreatic β-cells, this is the first report showing that palmitate regulates expression of numerous genes via direct molecular signalling mechanisms in liver cells.
Peer Reviewed Yes
Published Yes
Alternative URI
Volume 328
Issue Number 2
Page from 432
Page to 441
ISSN 0006-291X
Date Accessioned 2010-03-26
Language en_AU
Faculty Griffith Health Faculty
Subject Medicinal and Biomolecular Chemistry
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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