Rapid exposure assessment of PSII herbicides in surface water using a novel chlorophyll a fluorescence imaging assay

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Title Rapid exposure assessment of PSII herbicides in surface water using a novel chlorophyll a fluorescence imaging assay
Author Muller, Renee; Schreiber, Ulrich; Escher, Beate I.; Quayle, Pamela; Bengtson Nash, Susan; Mueller, Jochen F.
Journal Name Science of the Total Environment
Year Published 2008
Place of publication Netherlands
Publisher Elsevier
Abstract Recently a new Maxi-Imaging-PAM (Max-I-PAM) instrument for phytotoxicity assessment via chlorophyll fluorescence imaging was introduced. This new instrument allows rapid detection of the effects of PS II inhibiting herbicides which are high use agricultural chemicals frequently detected in surface waters in Australia and elsewhere. Several studies have applied the new instrument for detection of phytotoxicants in water using microalgae suspensions; however, these use preliminary protocols and to date no validated method is available for high throughput testing of environmental samples in 96-well plates. Here we developed and applied a new protocol allowing dose–response assessment of four samples within 2 h (8 dilutions in duplicate). The technique was found to be sensitive, with a detection limit of 2.3 ng l-1 for the herbicide diuron when testing solid phase extracts (SPE) of 1000 ml water samples, and reproducible both between experiments (coefficient of variation (CV) = 0.30) and within the 96-well plate (CV = 0.06). Relative potencies were determined for four reference PS II impacting herbicides (diuron > hexazinone > atrazine > simazine). Extracts from 1000 ml environmental samples and diuron spiked ultrapure water as well as passive sampler extracts were evaluated and good agreement was found between diuron equivalent concentrations calculated from bioassay results (DEQIPAM) and DEQCHEM values calculated from LCMS chemical analysis of the four reference compounds in the same samples. Overall, the technique provides a valuable bioanalytical tool for rapid and inexpensive effects-based assessment of PS II impacting herbicides in environmental mixtures.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1016/j.scitotenv.2008.02.062
Volume 401
Issue Number 1-3
Page from 51
Page to 59
ISSN 0048-9697
Date Accessioned 2010-07-01
Language en_US
Research Centre Environmental Futures Research Institute
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Environmental Sciences
URI http://hdl.handle.net/10072/33053
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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