Комплексный эколого-вирусологический мониторинг на территории Приморского края в 2003-2006 гг

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Title Комплексный эколого-вирусологический мониторинг на территории Приморского края в 2003-2006 гг
Author Shchelkanov, M. Yu; Ananyev, V. Yu.; Lvov, D. N.; Kireyev, D. Ye.; Guryev, Ye. L.; Akanina, D. S.; Galkina, I. V.; Aristova, V. A.; Moskvina, T. M.; Chumakov, V. M.; Baranov, N. I.; Gorelikov, V. N.; Usachev, Evgeny; Alkhovsky, S. V.; Lyapina, O. V.; Poglazov, А. В.; Shlyapnikova, O. V.; Burukhina, Ye. G.; Borisova, O. N.; Fedyakina, I. Т.; Burtseva, Ye. I.; Morozova, T. N.; Grenkova, Ye. P.; Grebennikova, T. V.; Prilipov, A. G.; Samokhvalov, Ye. I.; Zabarezhnyi, A. D.; Kolomeyets, S. A.; Miroshnikov, V. A.; Oropai, P. L.; Gaponov, V. V.; Semenov, V. L.; Suslov, I. O.; Volkov, V. A.; Yamnikova, S. S.; Aliper, T. I.; Dunayev, V. G.; Gromashevsky, V. L.; Maslov, D. V.; Novikov, F. Т.; Vlasov, N. A.; Deryabin, P. G.; Nepoklonov, Ye. A.; Zlobin, V. L.; Lvov, D. K.
Journal Name Voprosy Virusologii
Year Published 2007
Place of publication Russian Federation
Publisher Izdatel'stvo Meditsina
Abstract The paper presents the results of monitoring of viruses of Western Nile (WN), Japanese encephalitis (JE), tick-borne encephalitis (TBE), Geta, influenza A, as well as avian paramicroviruses type 1 (virus of Newcastle disease (ND)) and type 6 (APMV-6) in the Primorye Territory in 2003—2006. Totally throughout the period, specific antibodies to the viruses were detected by neutralization test in wild birds (7.3%, WN; 8.0%, Geta; 0.7% Batai; 2.8%, Alpine hare (Lepus timidus); by hemagglutination-inhibition test in cattle (11.4% WN; 5.9%, JE; j 3.0%, TBE; 11.6%, Geta), horses (6.1, 6.8, 0, and 25.3%, respectively), and pigs (5.4, 1.5, 0, and 5.9%, respectively) by enzyme immunoassay (IgG) in human beings (0.8, 0.5, 6.8, and 3.2%, respectively. Reverse-transcription polymerase chain reaction (RT-PCR) was used to reveal RNA of the NP segment of influenza A virus in 57.9 and 65% of the cloacal swabs from wild and domestic birds, respectively; and the HA-segment of subtype HH was not detected in 2005. HA/H5 RNA was recorded in 5.5 and 6.7% of the swabs from wild and domestic birds, respectively; 6% of the specimens from domestic birds were M-segment positive in 2006. RNA of influenza A virus NA/H7 and RNA was not detected throughout the years. In 2004, the cloacal swabs 8 isolated influenza A strains: two H3N8 and two H4N8 strains from European teals (Anas crecca), two (H3N8 and H6N2) strains from Baikal teals (A. formosa), one (H10N4) strain from shovelers (A. clypeata), and one (H4N8) from garganeys (A. querquedula). In 2004, one ND virus strain was isolated from the cloacal swabs from European teals (A. crecca). RT-PCR revealed RNA of this virus in some 8 more cloacal swabs from black ducks (A. poecilorhyncha) (3 positive specimens), pheasants (Phasianus colchicus) (n = 2), garganeys (A. querquedula) (n = 1), gadwalls (A. strepera) (n = 1), and geese (Anser anser domesticus) (n = 1). Sequencing of the 374-member fragment of the ND virus F gene, which included a proteolytic cleavage site, could assign two samples to the weakly pathogenetic variants of genotype 1, one sample to highly pathogenic variants of genotype 3a, five to highly pathogenic ones of genotype 5b. Isolation of APMV-6 (2003) from common egrets (Egretta alba) and geese (Ans. anser domesticus) is first described.
Peer Reviewed Yes
Published Yes
Volume 52
Issue Number 5
Page from 37
Page to 48
ISSN 0507-4088
Date Accessioned 2010-03-25
Language en_AU
Faculty Faculty of Science, Environment, Engineering and Technology
Subject PRE2009-Cross discipline
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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