Comparison of five in vitro bioassays to measure estrogenic activity in environmental waters

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Title Comparison of five in vitro bioassays to measure estrogenic activity in environmental waters
Author Leusch, Frederic; Jager, Chritsiaan de; Levi, Yves; Lim, Richard; Puijker, Leo; Sacher, Frank; Tremblay, Louis A.; Wilson, Vickie S.; Chapman, Heather Faye
Journal Name Environmental Science & Technology
Year Published 2010
Place of publication United States
Publisher American Chemical Society
Abstract Bioassays are well established in the pharmaceutical industry and single compound analysis, but there is still uncertainty about their usefulness in environmental monitoring.Wecompared the responses of five bioassays designed to measure estrogenic activity (the yeast estrogen screen, ER-CALUX, MELN,T47D-KBluc,andE-SCREENassays)andchemical analysis on extracts from four different water sources (groundwater, raw sewage, treated sewage, and river water). All five bioassays displayed similar trends and there was good agreement with analytical chemistry results. The data from the ER-CALUX and E-SCREEN bioassays were robust and predictable, and wellcorrelated with predictions from chemical analysis. The T47DKBluc appeared likewise promising, but with a more limited sample size it was less compelling. The YES assay was less sensitive than the other assays by an order of magnitude, which resulted in a larger number of nondetects. The MELN assay was less predictable, although the possibility that this was due to laboratory-specific difficulties cannot be discounted. With standardized bioassay data analysis and consistency of operating protocols, bioanalytical tools are a promising advance in the development of a tiered approach to environmental water quality monitoring.
Peer Reviewed Yes
Published Yes
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Copyright Statement Copyright 2010 American Chemical Society. Self-archiving of the author-manuscript version is not yet supported by this publisher. Please refer to the journal link for access to the definitive, published version or contact the author[s] for more information.
Volume 44
Issue Number 10
Page from 3853
Page to 3860
ISSN 0013-936X
Date Accessioned 2010-06-23
Language en_AU
Research Centre Australian Rivers Institute; Griffith Centre for Coastal Management
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Analytical Biochemistry; Environmental Monitoring
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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