The Effect of Laser Irradiation on Proliferation of Human Breast Carcinoma, Melanoma and Immortalized Mammary Epithelial Cells

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Title The Effect of Laser Irradiation on Proliferation of Human Breast Carcinoma, Melanoma and Immortalized Mammary Epithelial Cells
Author Powell, Katie; Low, Pauline; McDonnell, Ann; Laakso, Liisa; Ralph, Stephen John
Journal Name Photomedicine and Laser Surgery
Year Published 2010
Place of publication United States
Publisher Mary Ann Liebert, Inc.
Abstract Objective: This study compared the effects of different doses (J/cm2) of laser phototherapy at wavelengths of either 780, 830, or 904nm on human breast carcinoma, melanoma, and immortalized human mammary epithelial cell lines in vitro. In addition, we examined whether laser irradiation would malignantly transform the murine fibroblast NIH3T3 cell line. Background: Laser phototherapy is used in the clinical treatment of breast cancer-related lymphoedema, despite limited safety information. This study contributes to systematically developing guidelines for the safe use of laser in breast cancer-related lymphoedema. Methods: Human breast adenocarcinoma (MCF-7), human breast ductal carcinoma with melanomic genotypic traits (MDA-MB-435S), and immortalized human mammary epithelial (SVCT and Bre80hTERT) cell lines were irradiated with a single exposure of laser. MCF-7 cells were further irradiated with two and three exposures of each laser wavelength. Cell proliferation was assessed 24h after irradiation. Results: Although certain doses of laser increased MCF-7 cell proliferation, multiple exposures had either no effect or showed negative dose response relationships. No sign of malignant transformation of cells by laser phototherapy was detected under the conditions applied here. Conclusion: Before a definitive conclusion can be made regarding the safety of laser for breast cancer-related lymphoedema, further in vivo research is required.
Peer Reviewed Yes
Published Yes
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Copyright Statement Copyright 2010 Mary Ann Liebert, Inc., publishers. Self-archiving of the author-manuscript version is not yet supported by this publisher. Please refer to the journal link for access to the definitive, published version or contact the authors for more information.
Volume 28
Issue Number 1
Page from 115
Page to 123
ISSN 1549-5418
Date Accessioned 2010-03-18
Language en_AU
Research Centre Centre for Musculoskeletal Research; Menzies Health Institute Qld; Molecular Basis of Disease
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Biochemistry and Cell Biology
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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