Identification and characterization of the aspartate chemosensory receptor of Campylobacter jejuni

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Title Identification and characterization of the aspartate chemosensory receptor of Campylobacter jejuni
Author Hartley, Lauren; Shewell, Lucy Kathleen; Day, Christopher; Wilson, Jenny; Sandhu, Randeep; Ketley, Julian M.; Korolik, Victoria
Journal Name Molecular Microbiology
Year Published 2010
Place of publication United Kingdom
Publisher Wiley-Blackwell Publishing
Abstract Campylobacter jejuni is a highly motile bacterium that responds via chemotaxis to environmental stimuli to migrate towards favourable conditions. Previous in silico analysis of the C. jejuni strain NCTC11168 genome sequence identified 10 open reading frames, tlp1-10, that encode putative chemosensory receptors. We describe the characterization of the role and specificity of the Tlp1 chemoreceptor (Cj1506c). In vitro and in vivo models were used to determine if Tlp1 had a role in host colonization. The tlp1 - isogenic mutant was more adherent in cell culture, however, showed reduced colonization ability in chickens. Specific interactions between the purified sensory domain of Tlp1 and L-aspartate were identified using an amino acid array and saturation transfer difference nuclear magnetic resonance spectroscopy. Chemotaxis assays showed differences between migration of wild-type C. jejuni cells and that of a tlp1 - isogenic mutant, specifically towards aspartate. Furthermore, using yeast twohybrid and three-hybrid systems for analysis of protein–protein interactions, the cytoplasmic signalling domain of Tlp1 was found to preferentially interact with CheV, rather than the CheW homologue of the chemotaxis signalling pathway; this interaction was confirmed using immune precipitation assays. This is the first identification of an aspartate receptor in bacteria other than Escherichia coli and Salmonella enterica serovar Typhimurium.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1111/j.1365-2958.2009.07010.x
Volume 75
Issue Number 3
Page from 710
Page to 730
ISSN 0950-382X
Date Accessioned 2010-01-27
Language en_US
Research Centre Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Bacteriology
URI http://hdl.handle.net/10072/33172
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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