Confirming the 3D Solution Structure of a Short Double-Stranded DNA Sequence Using NMR Spectroscopy

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Title Confirming the 3D Solution Structure of a Short Double-Stranded DNA Sequence Using NMR Spectroscopy
Author Ruhayel, Rasha A.; Berners-Price, Sue
Journal Name Journal of Chemical Education
Year Published 2010
Place of publication United States
Publisher American Chemical Society
Abstract Nuclear magnetic resonance (NMR) spectroscopy is an indispensable tool used for the elucidation and confirmation of the solution structure of molecules that was first reported in 1938 by Isidor Rabi (1). A mere eight years later, its use was adapted for structure elucidation of liquids and solids by Felix Bloch and Edward Mills Purcell (2, 3), who four years later shared a Nobel Prize for their work. In more recent times, NMR spectroscopy has been extended to the study of larger biomolecules and has become a technique of paramount importance. In order to introduce students to this field of study, it is imperative that there be a sound understanding of how this technique works. Veeraraghavan describes a short course that effectively introduces students to the basics of NMR spectroscopy (4). A review of the articles available through this Journal shows examples of NMR studies of biological molecules such as peptides, phospholipids, and cytochrome c (5-8); however, there are no examples that use NMR spectroscopy to investigate DNA structure. The work herein represents a 6-h laboratory workshop with the aim of confirming the 3D structure of a short doublestrand DNA sequence, achieved using 2D 1H NOESY NMR spectroscopy, with the aid of a computer-generated model.
Peer Reviewed Yes
Published Yes
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Copyright Statement Copyright 2010 American Chemical Society. Self-archiving of the author-manuscript version is not yet supported by this publisher. Please refer to the journal link for access to the definitive, published version or contact the author[s] for more information.
Volume 87
Issue Number 7
Page from 732
Page to 734
ISSN 0021-9584
Date Accessioned 2010-07-23
Language en_AU
Research Centre Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Bioinorganic Chemistry
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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