Transcriptional Profiling Identifies the Metabolic Phenotype of Gonococcal Biofilms

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Title Transcriptional Profiling Identifies the Metabolic Phenotype of Gonococcal Biofilms
Author Falsetta, Megan L.; Bair, Thomas B.; Ku, Shan Chi; Hoven, Rachel N. vanden; Steichen, Christopher T.; McEwan, Alastair G.; Jennings, Michael Paul; Apicella, Michael A.
Journal Name Infection and Immunity
Year Published 2009
Place of publication United States
Publisher American Society for Microbiology
Abstract Neisseria gonorrhoeae, the etiologic agent of gonorrhea, is frequently asymptomatic in women, often leading to chronic infections. One factor contributing to this may be biofilm formation. N. gonorrhoeae can form biofilms on glass and plastic surfaces. There is also evidence that biofilm formation may occur during natural cervical infection. To further study the mechanism of gonococcal biofilm formation, we compared transcriptional profiles of N. gonorrhoeae biofilms to planktonic profiles. Biofilm RNA was extracted from N. gonorrhoeae 1291 grown for 48 h in continuous-flow chambers over glass. Planktonic RNA was extracted from the biofilm runoff. In comparing biofilm with planktonic growth, 3.8% of the genome was differentially regulated. Genes that were highly upregulated in biofilms included aniA, norB, and ccp. These genes encode enzymes that are central to anaerobic respiratory metabolism and stress tolerance. Downregulated genes included members of the nuo gene cluster, which encodes the proton-translocating NADH dehydrogenase. Furthermore, it was observed that aniA, ccp, and norB insertional mutants were attenuated for biofilm formation on glass and transformed human cervical epithelial cells. These data suggest that biofilm formation by the gonococcus may represent a response that is linked to the control of nitric oxide steady-state levels during infection of cervical epithelial cells.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1128/IAI.00036-09
Volume 77
Issue Number 9
Page from 3522
Page to 3532
ISSN 0019-9567
Date Accessioned 2010-02-18
Date Available 2010-09-10T05:18:05Z
Language en_AU
Research Centre Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Proteomics and Intermolecular Interactions (excl Medical Proteomics)
URI http://hdl.handle.net/10072/33882
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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