Thioredoxin Reductase is Essential for Protection of Neisseria gonorrhoeae against Killing by Nitric Oxide and for Bacterial Growth during Interaction with Cervical Epithelial Cells

There are no files associated with this record.

Title Thioredoxin Reductase is Essential for Protection of Neisseria gonorrhoeae against Killing by Nitric Oxide and for Bacterial Growth during Interaction with Cervical Epithelial Cells
Author Potter, Adam J.; Kidd, Stephen P.; Edwards, Jennifer L.; Falsetta, Megan L.; Apicella, Michael A.; Jennings, Michael Paul; McEwan, Alastair G.
Journal Name The Journal of Infectious Diseases
Year Published 2009
Place of publication United States
Publisher University of Chicago
Abstract In Neisseria gonorrhoeae, the MerR family transcription factor NmlR activates 3 operons in response to disulfide stress. In the present study, we show that trxB, a monocistronic operon under the control of NmlR, encodes a functional thioredoxin reductase. It is shown that neisserial TrxB has biochemical properties similar to those of its homologue from Escherichia coli. Analysis of a trxB mutant of N. gonorrhoeae showed that it was more sensitive to disulfide stress and to stress induced by organic hydroperoxides, superoxide, and nitric oxide than wild-type gonococcus. TrxB was found to be essential for the microaerobic induction of aniA and norB, the genes encoding nitrite reductase and nitric oxide reductase, respectively. The importance of TrxB during natural infection was demonstrated by the fact that the survival of gonococci within human cervical epithelial cells, as well as biofilm formation on these cells, was greatly reduced for a trxB mutant compared with a wild-type strain.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1086/595737
Volume 199
Issue Number 2
Page from 227
Page to 235
ISSN 0022-1899
Date Accessioned 2010-02-18
Date Available 2010-09-10T05:18:12Z
Language en_AU
Research Centre Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Infectious Agents
URI http://hdl.handle.net/10072/33884
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

Brief Record

Griffith University copyright notice