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dc.contributor.authorL. Varich, Natalia
dc.contributor.authorS. Kochergin-Nikitsky, Konstantin
dc.contributor.authorV. Usachev, Evgeny
dc.contributor.authorV. Usacheva, Olga
dc.contributor.authorG. Prilipov, Alexei
dc.contributor.authorG. Webster, Robert
dc.contributor.authorV. Kaverin, Nikolai
dc.date.accessioned2017-05-03T15:30:48Z
dc.date.available2017-05-03T15:30:48Z
dc.date.issued2009
dc.date.modified2010-09-15T07:05:29Z
dc.identifier.issn00221317
dc.identifier.doi10.1099/vir.0.010660-0
dc.identifier.urihttp://hdl.handle.net/10072/33957
dc.description.abstractThe locations of amino acid positions relevant to antigenic variation in the nucleoprotein (NP) of influenza virus are not conclusively known. We analysed the antigenic structure of influenza A virus NP by introducing site-specific mutations at amino acid positions presumed to be relevant for the differentiation of strain differences by anti-NP monoclonal antibodies. Mutant proteins were expressed in a prokaryotic system and analysed by performing ELISA with monoclonal antibodies. Four amino acid residues were found to determine four different antibody-binding sites. When mapped in a 3D X-ray model of NP, the four antigenically relevant amino acid positions were found to be located in separate physical sites of the NP molecule.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSociety for General Microbiology
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1730
dc.relation.ispartofpageto1733
dc.relation.ispartofissue7
dc.relation.ispartofjournalJournal of General Virology
dc.relation.ispartofvolume90
dc.rights.retentionY
dc.subject.fieldofresearchVirology
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchAgricultural and Veterinary Sciences
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode060506
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode07
dc.subject.fieldofresearchcode11
dc.titleLocation of antigenic sites recognized by monoclonal antibodies in the influenza A virus nucleoprotein molecule
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2009
gro.hasfulltextNo Full Text
gro.griffith.authorUsachev, Evgeny


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