Location of antigenic sites recognized by monoclonal antibodies in the influenza A virus nucleoprotein molecule

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Title Location of antigenic sites recognized by monoclonal antibodies in the influenza A virus nucleoprotein molecule
Author Varich, Natalia L.; Kochergin-Nikitsky, Konstantin S.; Usachev, Evgeny; Usacheva, Olga V.; Prilipov, Alexei G.; Webster, Robert G.; Kaverin, Nikolai V.
Journal Name Journal of General Virology
Year Published 2009
Place of publication United Kingdom
Publisher Society for General Microbiology
Abstract The locations of amino acid positions relevant to antigenic variation in the nucleoprotein (NP) of influenza virus are not conclusively known. We analysed the antigenic structure of influenza A virus NP by introducing site-specific mutations at amino acid positions presumed to be relevant for the differentiation of strain differences by anti-NP monoclonal antibodies. Mutant proteins were expressed in a prokaryotic system and analysed by performing ELISA with monoclonal antibodies. Four amino acid residues were found to determine four different antibody-binding sites. When mapped in a 3D X-ray model of NP, the four antigenically relevant amino acid positions were found to be located in separate physical sites of the NP molecule.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1099/vir.0.010660-0
Volume 90
Issue Number 7
Page from 1730
Page to 1733
ISSN 0022-1317
Date Accessioned 2010-02-27
Date Available 2010-09-15T07:05:29Z
Language en_AU
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Virology
URI http://hdl.handle.net/10072/33957
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1x

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