dc.contributor.author | L. Varich, Natalia | |
dc.contributor.author | S. Kochergin-Nikitsky, Konstantin | |
dc.contributor.author | V. Usachev, Evgeny | |
dc.contributor.author | V. Usacheva, Olga | |
dc.contributor.author | G. Prilipov, Alexei | |
dc.contributor.author | G. Webster, Robert | |
dc.contributor.author | V. Kaverin, Nikolai | |
dc.date.accessioned | 2017-05-03T15:30:48Z | |
dc.date.available | 2017-05-03T15:30:48Z | |
dc.date.issued | 2009 | |
dc.date.modified | 2010-09-15T07:05:29Z | |
dc.identifier.issn | 00221317 | |
dc.identifier.doi | 10.1099/vir.0.010660-0 | |
dc.identifier.uri | http://hdl.handle.net/10072/33957 | |
dc.description.abstract | The locations of amino acid positions relevant to antigenic variation in the nucleoprotein (NP) of influenza virus are not conclusively known. We analysed the antigenic structure of influenza A virus NP by introducing site-specific mutations at amino acid positions presumed to be relevant for the differentiation of strain differences by anti-NP monoclonal antibodies. Mutant proteins were expressed in a prokaryotic system and analysed by performing ELISA with monoclonal antibodies. Four amino acid residues were found to determine four different antibody-binding sites. When mapped in a 3D X-ray model of NP, the four antigenically relevant amino acid positions were found to be located in separate physical sites of the NP molecule. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Society for General Microbiology | |
dc.publisher.place | United Kingdom | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 1730 | |
dc.relation.ispartofpageto | 1733 | |
dc.relation.ispartofissue | 7 | |
dc.relation.ispartofjournal | Journal of General Virology | |
dc.relation.ispartofvolume | 90 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Virology | |
dc.subject.fieldofresearch | Biological Sciences | |
dc.subject.fieldofresearch | Agricultural and Veterinary Sciences | |
dc.subject.fieldofresearch | Medical and Health Sciences | |
dc.subject.fieldofresearchcode | 060506 | |
dc.subject.fieldofresearchcode | 06 | |
dc.subject.fieldofresearchcode | 07 | |
dc.subject.fieldofresearchcode | 11 | |
dc.title | Location of antigenic sites recognized by monoclonal antibodies in the influenza A virus nucleoprotein molecule | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.date.issued | 2009 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Usachev, Evgeny | |