Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae

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Title Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae
Author Wu, Hsing-Ju; Seib, Kate L.; Srikhanta, Yogitha Nageswary; Edwards, Jennifer; Kidd, Stephen P.; Maguire, Tina L.; Hamilton, Amanda; Pan, Kuan-Tin; Hsiao, He-Hsuan; Yao, Chen-Wen; Grimmond, Sean M.; Apicella, Michael A.; McEwan, Alastair G.; Wang, Andrew H-J.; Jennings, Michael Paul
Journal Name Journal of Proteomics
Year Published 2010
Place of publication Netherlands
Publisher Elsevier
Abstract Neisseria gonorrhoeae has evolved a complex and novel network of oxidative stress responses, including defence mechanisms that are dependent on manganese (Mn). We performed systematic analyses at the transcriptomic and proteomic (1D SDS-PAGE and Isotope-Coded Affinity Tag [ICAT]) levels to investigate the global expression changes that take place in a high Mn environment, which results in a Mn-dependent oxidative stress resistance phenotype. These studies revealed that there were proteins regulated at the post-transcriptional level under conditions of increased Mn concentration, including proteins involved in virulence (e.g., pilin, a key adhesin), oxidative stress defence (e.g., superoxide dismutase), cellular metabolism, protein synthesis, RNA processing and cell division. Mn regulation of inorganic pyrophosphatase (Ppa) indicated the potential involvement of phosphate metabolism in the Mn-dependent oxidative stress defence. A detailed analysis of the role of Ppa and polyphosphate kinase (Ppk) in the gonococcal oxidative stress response revealed that ppk and ppa mutant strains showed increased resistance to oxidative stress. Investigation of these mutants grown with high Mn suggests that phosphate and pyrophosphate are involved in Mn-dependent oxidative stress resistance.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1016/j.jprot.2009.12.001
Volume 73
Issue Number 5
Page from 899
Page to 916
ISSN 1874-3919
Date Accessioned 2010-07-16
Date Available 2010-10-06T06:56:16Z
Language en_AU
Research Centre Institute for Glycomics
Faculty Faculty of Science, Environment, Engineering and Technology
Subject Microbial Genetics
URI http://hdl.handle.net/10072/34411
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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