Microbiological pattern of arterial catheters in the intensive care unit

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Title Microbiological pattern of arterial catheters in the intensive care unit
Author Zhang, Li; Sriprakash, Kadaba Srinivasa; McMillan, David; Gowardman, John Robert; Patel, Bharat Kumar; Rickard, Claire
Journal Name BMC Microbiology
Year Published 2010
Place of publication United Kingdom
Publisher BioMed Central
Abstract Background: Intravascular catheter related infection (CRI) is one of the most serious nosocomial infections. Diagnostic criteria include a positive culture from the catheter tip along with blood, yet in many patients with signs of infection, current culture techniques fail to identify pathogens on catheter segments. We hypothesised that a molecular examination of the bacterial community on short term arterial catheters (ACs) would improve our understanding of the variety of organisms that are present in this niche environment and would help develop new methods for the diagnosis of CRI. Results: The whole bacterial community presenting on all ACs was evaluated by molecular methods, i.e., a strategy of whole community DNA extraction, PCR amplification followed by cloning and 16S rDNA sequence analysis. Ten ACs were removed from patients suspected of CRI and 430 clones from 5 “colonised” and 5 “uncolonised” (semiquantitative method) AC libraries were selected for sequencing and subsequent analysis. A total of 79 operational taxonomic units (OTUs) were identified at the level of 97% similarity belonging to six bacterial divisions. An average of 20 OTUs were present in each AC, irrespective of colonisation status. Conventional culture failed to reveal the majority of these bacteria. Conclusions: There was no significant difference in the bacterial diversity between the 'uncolonised' and 'colonised' ACs. This suggests that vascular devices cultured conventionally and reported as non infective may at times potentially be a significant source of sepsis in critically ill patients. Alternative methods may be required for the accurate diagnosis of CRI in critically ill patients.
Peer Reviewed Yes
Published Yes
Alternative URI http://dx.doi.org/10.1186/1471-2180-10-266
Copyright Statement Copyright 2010 Zhang, et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Volume 10
Page from 1
Page to 9
ISSN 1471-2180
Date Accessioned 2011-01-12
Language en_AU
Comments Page numbers are not for citation purposes. Instead, this article has the unique article number of 2010, 10:266.
Research Centre Centre for Health Practice Innovation; Menzies Health Institute Qld; Queensland Micro and Nanotechnology Centre
Faculty Griffith Health Faculty
Subject Infectious Diseases; Intensive Care; Medical Bacteriology
URI http://hdl.handle.net/10072/35526
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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