Exogenous Pyocyanin Alters Pseudomonas aeruginosa Susceptibility to Ciprofloxacin

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Title Exogenous Pyocyanin Alters Pseudomonas aeruginosa Susceptibility to Ciprofloxacin
Author Grant, Gary Dean; Zhang, TianTian; Gloyne, Lee; Perkins, Anthony Vincent; Kiefel, Milton; Dukie, Shailendra
Journal Name American Journal of Microbiology
Editor Dr. M. S. Ahmad
Year Published 2010
Place of publication United States
Publisher Science Publications
Abstract This in vitro cell-based study identified the contributing role of pyocyanin in the resistance of Pseudomonas aeruginosa to ciprofloxacin. Problem statement: P. aeruginosa is the major pathogen in the Cystic Fibrosis (CF) lung with pyocyanin being a critical component of its virulence. Prevalence is high and, once acquired, chronic infection is difficult to eliminate. Ciprofloxacin remains a crucial oral agent effective against P. aeruginosa, but resistance is increasingly reported. Approach:Here we examined the extent to which exogenously added pyocyanin affected P. aeruginosa susceptibility to ciprofloxacin and the contribution of altered efflux activity and biofilm production with the aim of ultimately increasing sensitivity. Results: Metabolic conversion of resazurin to resorufin was used as an index of bacterial cell growth while fluorescent measurement of acriflavine efflux and crystal violet staining was used as markers of efflux activity and biofilm production, respectively. Pyocyanin (100 μM) added exogenously decreased susceptibility of two P. aeruginosa strains, PAO1 and ATCC 27853 to ciprofloxacin at 125 and 500 mg L-1, respectively. Exogenously added pyocyanin decreased efflux activity in both strains while biofilm production was significantly increased. Conclusion: We conclude that increased biofilm production may contribute to the observed decreased susceptibility of P. aeruginosa to ciprofloxacin. Ciprofloxacin is a crucial orally effectively agent against P. aeruginosa with resistance being increasingly reported. This initial study highlights a potential mechanism that may underlie this resistance which may be of clinical interest. Further studies using additional antibiotics and the pyocyanin precursor 1-hydroxyphenazine are required.
Peer Reviewed Yes
Published Yes
Publisher URI http://www.thescipub.com/abstract/10.3844/ajmsp.2010.9.13
Copyright Statement Copyright remains with the authors 2010. The attached file is reproduced here in accordance with the copyright policy of the publisher. For information about this journal please refer to the journal's website or contact the authors.
Volume 1
Issue Number 1
Page from 9
Page to 13
ISSN 1948-982X
Date Accessioned 2011-01-09
Date Available 2011-07-12T07:52:18Z
Language en_AU
Research Centre Griffith Health Institute; Heart Foundation Research Centre; Institute for Glycomics; Molecular Basis of Disease
Faculty Griffith Health Faculty
Subject Basic Pharmacology
URI http://hdl.handle.net/10072/35654
Publication Type Journal Articles (Refereed Article)
Publication Type Code c1

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